Khristov S, Karadzhov I, Ignatov G, Khristova V
Vet Med Nauki. 1976;13(4):36-44.
Studied was the authors' inactivated polyvalent vaccine having an adjuvant, prepared from the respiratory viruses IBR, Pi-3, Ad-1 and Ad-3. It was found that at the subcutaneous vaccination of calves and pregnant cows there were virus-neutralizing, hemagglutination-inhibition, and complement-fixing antibodies as established by the 14th day following treatment. Revaccination was carried out on the twenty-eighth day when the antibodies reached high titers. The latter showed peak values on the 14th day following revaccination. The prevaccinal and vaccinal antibodies did not interfere with the process of immunogenesis. The virus-neutralizing antibodies against IBR had a titer of 5 log on the 150th day, while the virus-neutralizing and hemagglutination-inhibition antibodies against Pi-3 showed the same titer even after the 180th day following vaccination. There were in the milk of the vaccinated cows, in the seventh month of pregnancy, specific lactoglobulins of the IgA and IgG classes which were contained in the 27-36, 45-48 and 51-54 fractions, distributed in three peaks by their protein content. The level of the virus-neutralizing, hemagglutination-inhibition, and complement-fixing antibodies against the inactivated vaccinal respiratory viruses showed that the subcutaneous treatment with the polyvalent vaccine leads to the production of antibodies that are secreted with milk
对作者制备的含有佐剂的灭活多价疫苗进行了研究，该疫苗由呼吸道病毒传染性牛鼻气管炎病毒（IBR）、副流感3型病毒（Pi-3）、腺病毒1型（Ad-1）和腺病毒3型（Ad-3）制成。结果发现，在对犊牛和怀孕母牛进行皮下接种后，在治疗后的第14天就出现了病毒中和抗体、血凝抑制抗体和补体结合抗体。当抗体达到高滴度时，在第28天进行了再次接种。再次接种后，抗体滴度在第14天达到峰值。接种前和接种后的抗体均不干扰免疫发生过程。针对IBR的病毒中和抗体在第150天的滴度为5 log，而针对Pi-3的病毒中和抗体和血凝抑制抗体在接种后第180天仍保持相同滴度。在怀孕第七个月的接种母牛的乳汁中，IgA和IgG类的特异性乳球蛋白存在于27 - 36、45 - 48和51 - 54组分中，按蛋白质含量分布在三个峰值处。针对灭活疫苗呼吸道病毒的病毒中和抗体、血凝抑制抗体和补体结合抗体的水平表明，用多价疫苗进行皮下治疗可导致产生随乳汁分泌的抗体
