Khristov S, Karadzhov I
Vet Med Nauki. 1976;13(3):8-13.
Tested were the chemical and physico-chemical means for the inactivation of the infectious bovine rhinotracheitis virus. Formalin and saponin were shown to decrease the immunogenic activity of the virus. The inactivation of the virus, preserving its immunogenic properties, was achieved with the use of two inactivating factors (1 and 2), and the concentrated vaccines produced containing an oil adjuvant at amounts of 10 cu. cm per dose in the vaccination and revaccination at a 28-day interval proved highly immunogenic. Neutralizing antibodies in the vaccinated rabbits and calves were demonstrated after the 14th day following treatment. The antibody titers on the 28th day following vaccination, and on the 14th day following revaccination reached their peak levels: log 2.5 to 4.5 and 6.5 to 7.5, respectively. The problem is discussed of the correlation between the circulation and the secretory neutralizing antibodies after immunization with an inactivated IBR vaccine.
对传染性牛鼻气管炎病毒失活的化学和物理化学方法进行了测试。结果表明，福尔马林和皂角苷会降低病毒的免疫原活性。通过使用两种灭活因子（1和2）实现了病毒的失活，同时保留其免疫原特性，并且所生产的浓缩疫苗含有油佐剂，每剂量为10立方厘米，在间隔28天进行的初次免疫和再次免疫中证明具有高度免疫原性。在治疗后的第14天，接种疫苗的兔子和小牛体内出现了中和抗体。接种疫苗后第28天以及再次接种后第14天的抗体滴度达到峰值水平：分别为log 2.5至4.5和6.5至7.5。文中讨论了用灭活的传染性牛鼻气管炎疫苗免疫后循环中和抗体与分泌性中和抗体之间的相关性问题。
