Ivey D M, Krulwich T A
Department of Biochemistry, Mount Sinai School of Medicine, City University of New York, NY 10029.
Mol Gen Genet. 1991 Oct;229(2):292-300. doi: 10.1007/BF00272169.
The atp operon from the extreme alkaliphile Bacillus firmus OF4 was cloned and sequenced, and shown to contain genes for the eight structural subunits of the ATP synthase, preceded by a ninth gene predicted to encode a 14 kDa hydrophobic protein. The arrangement of genes is identical to that of the atp operons from Escherichia coli, Bacillus megaterium, and thermophilic Bacillus PS3. The deduced amino acid sequences of the subunits of the enzyme are also similar to their homologs in other ATP synthases, except for several unusual substitutions, particularly in the a and c subunits. These substitutions are in domains that have been implicated in the mechanism of proton translocation through F0-ATPase, and therefore could contribute to the gating properties of the alkaliphile ATP synthase or its capacity for proton capture.
来自极端嗜碱菌坚强芽孢杆菌OF4的atp操纵子被克隆并测序，结果显示它包含ATP合酶八个结构亚基的基因，前面还有一个预计编码14 kDa疏水蛋白的第九个基因。基因排列与大肠杆菌、巨大芽孢杆菌和嗜热芽孢杆菌PS3的atp操纵子相同。该酶亚基的推导氨基酸序列也与其在其他ATP合酶中的同源物相似，但有几个不寻常的取代，特别是在a和c亚基中。这些取代位于与质子通过F0 - ATP酶转运机制有关的结构域中，因此可能有助于嗜碱菌ATP合酶的门控特性或其质子捕获能力。
