酵母表面展示技术十年：我们如今处于何方？
A decade of yeast surface display technology: where are we now?
作者信息
Pepper Lauren R, Cho Yong Ku, Boder Eric T, Shusta Eric V
机构信息
Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, USA.
出版信息
Comb Chem High Throughput Screen. 2008 Feb;11(2):127-34. doi: 10.2174/138620708783744516.
Abstract
Yeast surface display has become an increasingly popular tool for protein engineering and library screening applications. Recent advances have greatly expanded the capability of yeast surface display, and are highlighted by cell-based selections, epitope mapping, cDNA library screening, and cell adhesion engineering. In this review, we discuss the state-of-the-art yeast display methodologies and the rapidly expanding set of applications afforded by this technology.
摘要
酵母表面展示已成为蛋白质工程和文库筛选应用中越来越受欢迎的工具。最近的进展极大地扩展了酵母表面展示的能力，其亮点包括基于细胞的筛选、表位作图、cDNA文库筛选和细胞黏附工程。在本综述中，我们讨论了最先进的酵母展示方法以及该技术带来的迅速扩展的一系列应用。
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