Kusanagi Hiroaki, Hida Akiko, Satoh Kohtoku, Echizenya Masaru, Shimizu Tetsuo, Pendergast Julie S, Yamazaki Shin, Mishima Kazuo
Division of Neuropsychiatry, Department of Neuro and Locomotor Science, Akita University School of Medicine, Akita, Japan.
Neurosci Res. 2008 Jun;61(2):136-42. doi: 10.1016/j.neures.2008.01.012. Epub 2008 Feb 6.
The circadian clock system regulates daily rhythms of physiology and behavior. The mammalian master clock in the suprachiasmatic nuclei orchestrates these biological rhythms in peripheral tissues. Since blood is the most accessible tissue source, we sought to dissect the human circadian clock system by characterizing clock gene expression in human peripheral blood mononuclear cells (PBMCs) isolated from eight young, healthy subjects. By evaluating the temporal expression profiles of 10 circadian clock genes, we found that Period 1 (Per1), Per2, and Per3 are rhythmically expressed in human blood samples. Our results suggest that evaluating the rhythmic expression of human Per genes could reveal an individual's circadian phenotype.
昼夜节律时钟系统调节生理和行为的日常节律。视交叉上核中的哺乳动物主时钟协调外周组织中的这些生物节律。由于血液是最容易获取的组织来源，我们试图通过表征从八名年轻健康受试者分离出的人外周血单核细胞（PBMC）中的时钟基因表达来剖析人类昼夜节律时钟系统。通过评估10个昼夜节律时钟基因的时间表达谱，我们发现周期蛋白1（Per1）、Per2和Per3在人类血液样本中有节律地表达。我们的结果表明，评估人类Per基因的节律性表达可以揭示个体的昼夜节律表型。
