Andrew and Peggy Cherng Department of Medical Engineering, California Institute of Technology, Pasadena, CA, USA.
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Sci Rep. 2020 Nov 25;10(1):20540. doi: 10.1038/s41598-020-77073-3.
An individual's immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a platform that leverages multiplexed single-cell sequencing and out-of-clinic capillary blood extraction to enable simplified, cost-effective profiling of the human immune system across people and time at single-cell resolution. Using the platform, we detect widespread differences in cell type-specific gene expression between subjects that are stable over multiple days.
个体的免疫系统受到遗传和环境因素的共同驱动，且这些因素随时间而变化。为了更好地了解不同免疫细胞类型中基因表达的时间和个体间可变性，我们开发了一个平台，该平台利用多重单细胞测序和非住院毛细血管血提取，以单细胞分辨率在个体和时间上简化和降低成本来对人类免疫系统进行分析。使用该平台，我们检测到了受试者之间细胞类型特异性基因表达的广泛差异，这些差异在多天内是稳定的。
