Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
Med Sci Monit. 2011 Apr;17(4):BR110-5. doi: 10.12659/msm.881711.
Doxorubicin (DOX) is a commonly used chemotherapeutic agent. It is associated with serious dose-limiting cardiotoxicity, which is at least partly caused by generation of reactive oxygen species (ROS). Supplementations with bilberries were effective in reducing oxidative stress in many tissue injuries due their high content of antioxidants. The present study investigated the potential protective effect of bilberry extract against DOX-induced cardiotoxicity in rats.
MATERIAL/METHODS: Rats were treated orally with a methanolic extract of bilberry for 10 days. DOX was injected intraperitoneally on day 7. Twenty-four hours after the last bilberry administration, rats were subjected to ECG study. Blood was then withdrawn and cardiac tissues were dissected for assessment of oxidative stress and cardiac tissue injury. Cardiac tissues were also subjected to histopathological examination.
Bilberry extract significantly inhibited DOX-provoked reduced glutathione depletion and accumulation of oxidized glutathione, malondialdehyde and protein carbonyls in cardiac tissues. This was accompanied by significant amelioration of reduced cardiac catalase, superoxide dismutase, and glutathione peroxidase activities; and increased cardiac myeloperoxidase activity in response to DOX challenge. Pretreatment with bilberry significantly guarded against DOX-induced increase in serum activities of lactate dehydrogenase, creatine phosphokinase and creatine kinase-MB, as well as the level of troponin I. Bilberry alleviated ECG changes in rats treated with DOX and attenuated its pathological changes.
Bilberry protects against DOX-induced cardiotoxicity in rats. This can be attributed, at least in part, to its antioxidant activity.
多柔比星（DOX）是一种常用的化疗药物。它与严重的剂量限制的心脏毒性有关，这至少部分是由活性氧（ROS）的产生引起的。由于富含抗氧化剂，越桔补充剂在许多组织损伤中减轻氧化应激的效果非常显著。本研究旨在探讨越桔提取物对大鼠多柔比星诱导的心脏毒性的潜在保护作用。
材料/方法：大鼠连续 10 天口服越桔甲醇提取物。第 7 天腹腔注射多柔比星。最后一次给予越桔提取物 24 小时后，对大鼠进行心电图研究。然后抽取血液并分离心脏组织，以评估氧化应激和心脏组织损伤。心脏组织还进行了组织病理学检查。
越桔提取物显著抑制 DOX 引起的心脏组织中还原型谷胱甘肽的消耗和氧化型谷胱甘肽、丙二醛和蛋白质羰基的积累。这伴随着心脏过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶活性的显著改善；以及心脏髓过氧化物酶活性的增加，以应对 DOX 的挑战。越桔预处理显著防止了 DOX 引起的血清乳酸脱氢酶、肌酸磷酸激酶和肌酸激酶同工酶-MB 活性的增加，以及肌钙蛋白 I 水平的升高。越桔减轻了 DOX 处理大鼠的心电图变化，并减轻了其病理变化。
越桔可预防大鼠多柔比星诱导的心脏毒性。这至少部分归因于其抗氧化活性。
