Konishi E, Pincus S, Fonseca B A, Shope R E, Paoletti E, Mason P W
Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06510.
Virology. 1991 Nov;185(1):401-10. doi: 10.1016/0042-6822(91)90788-d.
Immunization with recombinant vaccinia viruses that specified the synthesis of Japanese encephalitis virus (JEV) glycoproteins protected mice from a lethal intraperitoneal challenge with JEV. Recombinants which coexpressed the genes for the structural glycoproteins, prM and E, elicited high levels of neutralizing (NEUT) and hemagglutination inhibiting (HAI) antibodies in mice and protected mice from a lethal challenge by JEV. Recombinants expressing only the gene for the nonstructural glycoprotein, NS1, induced antibodies to NS1 but provided low levels of protection from a similar challenge dose of JEV. Antibodies to the NS3 protein in postchallenge sera, representing the degree of infection with challenge virus, were inversely correlated to NEUT and HAI titers and levels of protection. These results indicate that although vaccinia recombinants expressing NS1 can provide some protection from lethal JEV infection, recombinants expressing prM and E elicited higher levels of protective immunity.
用能合成日本脑炎病毒（JEV）糖蛋白的重组痘苗病毒进行免疫接种，可保护小鼠免受JEV致死性腹腔内攻击。共表达结构糖蛋白prM和E基因的重组体在小鼠体内引发了高水平的中和（NEUT）抗体和血凝抑制（HAI）抗体，并保护小鼠免受JEV的致死性攻击。仅表达非结构糖蛋白NS1基因的重组体诱导产生了针对NS1的抗体，但对相似攻击剂量的JEV提供的保护水平较低。攻击后血清中针对NS3蛋白的抗体代表了感染攻击病毒的程度，与NEUT和HAI滴度及保护水平呈负相关。这些结果表明，尽管表达NS1的痘苗重组体可提供一定程度的保护，使其免受致死性JEV感染，但表达prM和E的重组体引发了更高水平的保护性免疫。
