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口服接种编码鸭坦布苏病毒衣壳蛋白的 DNA 疫苗可诱导保护免疫。

Oral Vaccination with a DNA Vaccine Encoding Capsid Protein of Duck Tembusu Virus Induces Protection Immunity.

机构信息

Research Center of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Wenjiang District, Chengdu 611130, China.

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang District, Chengdu 611130, China.

出版信息

Viruses. 2018 Apr 6;10(4):180. doi: 10.3390/v10040180.

DOI:10.3390/v10040180
PMID:29642401
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5923474/
Abstract

The emergence of duck tembusu virus (DTMUV), a new member of the genus, has caused great economical loss in the poultry industry in China. Since the outbreak and spread of DTMUV is hard to control in a clinical setting, an efficient and low-cost oral delivery DNA vaccine SL7207 (pVAX1-C) based on the capsid protein of DTMUV was developed and evaluated in this study. The antigen capsid protein was expressed from the DNA vaccine SL7207 (pVAX1-C), both in vitro and in vivo. The humoral and cellular immune responses in vivo were observed after oral immunization with the SL7207 (pVAX1-C) DNA vaccine. High titers of the specific antibody against the capsid protein and the neutralizing antibody against the DTMUV virus were both detected after inoculation. The ducks were efficiently protected from lethal DTMUV exposure by the SL7207 (pVAX1-C) vaccine in this experiment. Taken together, we demonstrated that the capsid protein of DTMUV possesses a strong immunogenicity against the DTMUV infection. Moreover, an oral delivery of the DNA vaccine SL7207 (pVAX1-C) utilizing SL7207 was an efficient way to protect the ducks against DTMUV infection and provides an economic and fast vaccine delivery strategy for a large scale clinical use.

摘要

鸭坦布苏病毒（DTMUV）的出现是属中的一个新成员，在中国的家禽业造成了巨大的经济损失。由于在临床环境中难以控制 DTMUV 的爆发和传播，因此本研究开发并评估了一种基于 DTMUV 衣壳蛋白的高效且低成本的口服递送 DNA 疫苗 SL7207（pVAX1-C）。抗原衣壳蛋白由 DNA 疫苗 SL7207（pVAX1-C）在体外和体内表达。通过口服免疫 SL7207（pVAX1-C）DNA 疫苗观察体内的体液和细胞免疫反应。接种后均检测到针对衣壳蛋白的特异性抗体和针对 DTMUV 病毒的中和抗体的高滴度。在本实验中，SL7207（pVAX1-C）疫苗可有效保护鸭子免受致死性 DTMUV 暴露。综上所述，我们证明了 DTMUV 的衣壳蛋白对 DTMUV 感染具有很强的免疫原性。此外，利用 SL7207 进行口服递送 DNA 疫苗 SL7207（pVAX1-C）是保护鸭子免受 DTMUV 感染的有效方法，为大规模临床应用提供了经济快速的疫苗接种策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d71/5923474/730fe499558e/viruses-10-00180-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d71/5923474/e39d7efa86e8/viruses-10-00180-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d71/5923474/fcf5bf474cf9/viruses-10-00180-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d71/5923474/bfaf0bb8bcde/viruses-10-00180-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d71/5923474/730fe499558e/viruses-10-00180-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d71/5923474/e39d7efa86e8/viruses-10-00180-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d71/5923474/fcf5bf474cf9/viruses-10-00180-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d71/5923474/bfaf0bb8bcde/viruses-10-00180-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d71/5923474/730fe499558e/viruses-10-00180-g004.jpg

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An updated review of avian-origin Tembusu virus: a newly emerging avian Flavivirus.禽源坦布苏病毒最新综述：一种新出现的禽黄病毒
J Gen Virol. 2017 Oct;98(10):2413-2420. doi: 10.1099/jgv.0.000908. Epub 2017 Sep 6.
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Efficacy assessment of an inactivated Tembusu virus vaccine candidate in ducks.
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