Fuentes Esquivel Diana Berenice, Pérez Betsabé Rodríguez, Betancourt Nelly Tovar, García Tovar Carlos Gerardo, Penieres Carrillo José Guillermo, Galindo Florentina Hernández, Flores Javier Pérez, Sánchez Tonatiuh Alejandro Cruz
Laboratorio de Servicio de Análisis de Propóleos (LASAP®), Facultad de Estudios Superiores Cuautitlán, Campo 4, National Autonomous University of México, Km 2.5 Carretera Cuautitlán-Teoloyucan, San Sebastián Xhala, Cuautitlán Izcalli 54714, State of México, Mexico.
Laboratorio de Morfología Veterinaria y Biología Celular, Facultad de Estudios Superiores Cuautitlán, Campo 4, National Autonomous University of México, Km 2.5 Carretera Cuautitlán-Teoloyucan, San Sebastián Xhala, Cuautitlán Izcalli 54714, State of México, Mexico.
Vet Sci. 2024 Feb 28;11(3):106. doi: 10.3390/vetsci11030106.
This work describes the antimycotic activity of propolis from the stingless bees and , collected from two Mexican regions (Veracruz and Chiapas, respectively), against three clinical isolates and the reference strain ATCC 14522 of , the causative agent of canine otitis. The chemical components of the ethanolic extracts of propolis were determined by gas chromatography coupled with mass spectrometry (GC-MS), and sesquiterpenes were the predominant compounds. The antimycotic activity was evaluated by plate microdilution. The induced changes in the yeasts were evaluated by fluorescence microscopy and staining with calcofluor white and propidium iodide. The minimum inhibitory concentration (MIC) was 7.11 mg/mL, and the minimum fungicidal concentration was 21.33 mg/mL for both extracts. The EPPs of and caused substantial damage to yeast morphology, where the propidium iodide staining of the yeasts treated with both EEPs revealed the penetration of this marker, which indicates the destruction of the cell wall and plasma membrane of the fungi. This result suggests that these types of propolis could be used as alternative treatments for canine external otitis. To the best of our knowledge, this seems to be the first scientific report that has demonstrated structural damage in by Mexican stingless bee propolis.
这项研究描述了分别从墨西哥两个地区（韦拉克鲁斯州和恰帕斯州）采集的无刺蜂蜂胶对犬中耳炎病原体、三种临床分离株及参考菌株ATCC 14522的抗真菌活性。采用气相色谱 - 质谱联用（GC - MS）法测定蜂胶乙醇提取物的化学成分，倍半萜类化合物是主要成分。通过平板微量稀释法评估抗真菌活性。利用荧光显微镜以及用荧光增白剂和碘化丙啶染色来评估酵母细胞的诱导变化。两种提取物的最低抑菌浓度（MIC）均为7.11 mg/mL，最低杀菌浓度均为21.33 mg/mL。两种蜂胶乙醇提取物（EEPs）对酵母形态造成了严重破坏，用两种EEPs处理后的酵母经碘化丙啶染色显示该标记物的渗透，这表明真菌的细胞壁和质膜遭到破坏。该结果表明，这些类型的蜂胶可作为犬外耳炎的替代治疗方法。据我们所知，这似乎是第一份证明墨西哥无刺蜂蜂胶对[此处原文可能有缺失，未明确具体所指真菌名称]造成结构损伤的科学报告。
