Pharmacognosy Laboratory, Biotechnology and Prototypes Unit, Faculty of Higher Studies Iztacala, National Autonomous University of Mexico, Av. de los Barrios No. 1, Los Reyes Iztacala, Tlalnepantla, Edo. de México, C.P. 54090, Mexico.
Biomedical Research Laboratory in Natural Products, Medicine Career, Faculty of Higher Studies Iztacala, National Autonomous University of Mexico, Avenida de los Barrios Numero 1, Colonia Los Reyes Iztacala, Tlalnepantla, Edo. de Mexico, C.P. 54090, Mexico.
BMC Microbiol. 2023 Nov 4;23(1):325. doi: 10.1186/s12866-023-03064-9.
This research evaluated the anti-Candida albicans effect of Mexican propolis from Chihuahua. Chemical composition of the ethanolic extract of propolis was determined by GC-MS, HPLC-DAD, and HPLC-MS. The presence of anthraquinone, aromatic acid, fatty acids, flavonoids, and carbohydrates was revealed.
The anti-Candida activity of propolis was determined. The inhibitions halos were between 10.0 to 11.8 mm; 25% minimum inhibitory concentration (0.5 mg/ml) was fungistatic, and 50% minimum inhibitory concentration (1.0 mg/ml) was fungicidal. The effect of propolis on the capability of C. albicans to change its morphology was evaluated. 25% minimum inhibitory concentration inhibited to 50% of germ tube formation. Staining with calcofluor-white and propidium iodide was performed, showing that the propolis affected the integrity of the cell membrane. INT1 gene expression was evaluated by qRT-PCR. Propolis significantly inhibited the expression of the INT1 gene encodes an adhesin (Int1p). Chihuahua propolis extract inhibited the proliferation of Candida albicans, the development of the germ tube, and the synthesis of adhesin INT1.
Given the properties demonstrated for Chihuahua propolis, we propose that it is a candidate to be considered as an ideal antifungal agent to help treat this infection since it would not have the toxic effects of conventional antifungals.
本研究评估了来自奇瓦瓦的墨西哥蜂胶的抗白色念珠菌作用。通过 GC-MS、HPLC-DAD 和 HPLC-MS 确定了蜂胶乙醇提取物的化学成分。揭示了蒽醌、芳香酸、脂肪酸、类黄酮和碳水化合物的存在。
确定了蜂胶的抗真菌活性。抑菌环在 10.0 至 11.8 毫米之间；25%最小抑菌浓度（0.5 毫克/毫升）具有抑菌作用，而 50%最小抑菌浓度（1.0 毫克/毫升）具有杀菌作用。评估了蜂胶对白色念珠菌改变形态能力的影响。25%最小抑菌浓度抑制 50%的芽管形成。用钙荧光白和碘化丙啶染色，表明蜂胶影响了细胞膜的完整性。通过 qRT-PCR 评估了 INT1 基因的表达。蜂胶显著抑制了编码黏附素（Int1p）的 INT1 基因的表达。奇瓦瓦蜂胶提取物抑制白色念珠菌的增殖、芽管的发育和黏附素 INT1 的合成。
鉴于奇瓦瓦蜂胶表现出的特性，我们建议将其作为一种理想的抗真菌剂来考虑，以帮助治疗这种感染，因为它不会产生传统抗真菌药物的毒副作用。
