O'Donoghue Keelin, Sultan Hanan A, Al-Allaf Faisal A, Anderson Jonathan R, Wyatt-Ashmead Josephine, Fisk Nicholas M
Institute of Reproductive and Developmental Biology, Division of Surgery, Oncology, Reproduction and Anaesthesia, Faculty of Medicine, Imperial College London, London, UK.
Reprod Biomed Online. 2008 Mar;16(3):382-90. doi: 10.1016/s1472-6483(10)60600-1.
Fetal cells trafficking into maternal blood during pregnancy engraft tissues and persist for decades in marrow and bone. While persistent fetal cells were initially implicated in autoimmune disease, animal studies suggest that microchimeric fetal cells play a broader role in response to tissue injury. This study investigated whether fetal cells participate in tissue repair after human pregnancy. Specimens were obtained from women undergoing surgery for suspected lung cancer. Y-fluorescence in-situ hybridization was performed on paraffin-embedded sections, with the investigator blinded to medical histories. Male cells were identified in lung/thymus tissue from all women with known male pregnancies, but not in those without sons. The frequency of male microchimeric cells was seven-fold greater in lung/thymus tissues than marrow and was two-fold greater than normal bone from the same women. Nested-polymerase chain reaction for sex determining region Y confirmed male DNA in tissues. Male cells in lung were clustered in tumour rather than surrounding healthy tissues. In conclusion, male presumed-fetal cells were identified in pathological post-reproductive tissues, where they were more likely to be located in diseased tissues at several-fold higher frequency than normal tissues. It is suggested that fetal cells are present at sites of tissue injury and may be stem cells, either recruited from marrow or having proliferated locally.
孕期进入母体血液的胎儿细胞会植入组织，并在骨髓和骨骼中持续存在数十年。虽然最初认为持续存在的胎儿细胞与自身免疫性疾病有关，但动物研究表明，微嵌合胎儿细胞在对组织损伤的反应中发挥着更广泛的作用。本研究调查了胎儿细胞是否参与人类妊娠后的组织修复。标本取自因疑似肺癌接受手术的女性。对石蜡包埋切片进行Y荧光原位杂交，研究者对病史不知情。在所有已知怀有男性胎儿的女性的肺/胸腺组织中均鉴定出男性细胞，而在没有儿子的女性中则未发现。肺/胸腺组织中男性微嵌合细胞的频率比骨髓高7倍，比同一女性的正常骨骼高2倍。用于Y染色体性别决定区的巢式聚合酶链反应证实了组织中的男性DNA。肺中的男性细胞聚集在肿瘤中而非周围的健康组织中。总之，在生殖后的病理组织中鉴定出了推测为胎儿的男性细胞，它们更有可能以比正常组织高几倍的频率位于患病组织中。提示胎儿细胞存在于组织损伤部位，可能是从骨髓募集或在局部增殖的干细胞。
