Ando S, Suwa T, Takashima I, Hashimoto N
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
J Vet Med Sci. 1991 Aug;53(4):691-7. doi: 10.1292/jvms.53.691.
The kinetics of the antigen production of Chlamydia psittaci strains Izawa-1 and Pigeon-1041 (P-1041) was examined every 6 hr after infection up to 48 hr, by the indirect immunofluorescent antibody technique using monoclonal antibodies (MAbs). All three genus-specific antigenic determinants on lipopolysaccharide (LPS) appeared during the whole growth cycle. Antigenic determinants on proteins were, on the other hand, detected at various time periods from the early to the late stages of infection. However, a cross-reactive antigenic determinant on protein recognized by a MAb 3E9 was also detected during the whole growth cycle, similar to that on LPS. The time of appearance of common antigenic determinants on proteins of Izawa-1 and P-1041 was examined using cross-reactive MAbs, and it varied depending on heterologous and homologous MAbs. From the relationship between the detection of antigenic determinants and the morphological changes of chlamydial particles revealed by electron microscopy during the growth cycle, the antigenic determinants on proteins of Chlamydia psittaci were divided into two groups; one was specific to the elementary body and the other was coexisting in both the elementary body and the reticulate body.
利用单克隆抗体（MAb）通过间接免疫荧光抗体技术，在感染后每6小时检测一次鹦鹉热衣原体菌株Izawa-1和鸽子-1041（P-1041）的抗原产生动力学，直至48小时。脂多糖（LPS）上的所有三种属特异性抗原决定簇在整个生长周期中均出现。另一方面，蛋白质上的抗原决定簇在感染的早期到晚期的不同时间段被检测到。然而，与LPS上的情况类似，在整个生长周期中也检测到了单克隆抗体3E9识别的蛋白质上的交叉反应性抗原决定簇。使用交叉反应性单克隆抗体检测了Izawa-1和P-1041蛋白质上共同抗原决定簇的出现时间，其因异源和同源单克隆抗体而异。从生长周期中抗原决定簇的检测与电子显微镜揭示的衣原体颗粒形态变化之间的关系来看，鹦鹉热衣原体蛋白质上的抗原决定簇分为两组；一组是原体特异性的，另一组在原体和网状体中都存在。
