Jiménez Jesús Angel, Alonso-Ramírez Ana, Nicolás Carlos
Departamento de Fisiología Vegetal, Centro Hispano-Luso de Investigaciones Agrarias, Facultad de Biología, Universidad de Salamanca, Plaza de los Doctores de la Reina s/n, 37007 Salamanca, Spain.
J Plant Physiol. 2008 Nov 28;165(17):1798-807. doi: 10.1016/j.jplph.2007.11.013. Epub 2008 Mar 17.
Two abscisic acid (ABA)-responsive clones (FsDhn1 and FsClo1) were isolated from a cDNA library of ABA-treated seeds of Fagus sylvatica L. FsDhn1 codes for type-II late embryogenesis abundant (LEA) proteins, also known as dehydrins. The corresponding transcripts were ABA-induced and expressed when seeds were artificially dried. FsClo1 codes for a caleosin, a calcium-binding protein. By expressing FsCLO1 as a fusion protein in Escherichia coli, we obtained biochemical evidence of its ability to bind calcium, a second messenger involved in ABA signaling. This cDNA clone is seed specific, shows a LEA expression pattern and is also induced by ABA and dehydration treatments. The relationship of both genes with seed responses to ABA is discussed.
从欧洲水青冈经脱落酸（ABA）处理的种子cDNA文库中分离出两个ABA反应克隆（FsDhn1和FsClo1）。FsDhn1编码II型胚胎后期丰富蛋白（LEA），也称为脱水蛋白。相应的转录本受ABA诱导，在种子人工干燥时表达。FsClo1编码一种钙结合蛋白caleosin。通过在大肠杆菌中表达FsCLO1融合蛋白，我们获得了其结合钙的生化证据，钙是ABA信号传导中的第二信使。该cDNA克隆具有种子特异性，呈现LEA表达模式，也受ABA和脱水处理诱导。文中讨论了这两个基因与种子对ABA反应的关系。
