Murata Takahiko, Ohtsuka Chigumi, Terayama Yasuo
Department of Neurology, Iwate Prefectural Ninohe Hospital, Ninohe, Iwate, Japan.
Free Radic Res. 2008 Mar;42(3):221-5. doi: 10.1080/10715760701877262.
To investigate the possibility that mitochondrial oxidative damage or oxidative DNA damage or both contribute to the neurodegenerative process of sporadic amyotrophic lateral sclerosis (sALS), this study used high-performance liquid chromatography with an electrochemical detector to measure the concentrations of the reduced and oxidized forms of coenzyme Q10 (CoQ10) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the cerebrospinal fluid (CSF) of 17 patients with sALS and 17 age-matched controls with no neurological diseases. The percentage of oxidized CoQ10 in the CSF of sALS patients was greater than that in the CSF of controls (p<0.002) and was negatively correlated with the duration of illness (rho=-0.61, p<0.01). The concentration of 8-OHdG in the CSF of sALS patients was greater than that in the CSF of controls (p<0.005) and was positively correlated with the duration of illness (rho=0.53, p<0.005). The percentage of oxidized CoQ10 was correlated with the concentrations of 8-OHdG in the CSF of sALS patients (rho=-0.53, p<0.05). These results suggest that both mitochondrial oxidative damage and oxidative DNA damage play important roles in the pathogenesis of sporadic amyotrophic lateral sclerosis.
为了研究线粒体氧化损伤或氧化性DNA损伤或两者共同作用是否导致散发性肌萎缩侧索硬化症（sALS）的神经退行性变过程，本研究采用高效液相色谱-电化学检测器来测量17例sALS患者和17例年龄匹配的无神经疾病对照者脑脊液（CSF）中辅酶Q10（CoQ10）还原型和氧化型以及8-羟基-2'-脱氧鸟苷（8-OHdG）的浓度。sALS患者脑脊液中氧化型CoQ10的百分比高于对照组脑脊液中的百分比（p<0.002），且与病程呈负相关（rho=-0.61，p<0.01）。sALS患者脑脊液中8-OHdG的浓度高于对照组脑脊液中的浓度（p<0.005），且与病程呈正相关（rho=0.53，p<0.005）。sALS患者脑脊液中氧化型CoQ10的百分比与8-OHdG的浓度相关（rho=-0.53，p<0.05）。这些结果表明，线粒体氧化损伤和氧化性DNA损伤在散发性肌萎缩侧索硬化症的发病机制中均起重要作用。
