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一种用于诊断血吸虫病的新型毛蚴孵化装置。

A new miracidia hatching device for diagnosing schistosomiasis.

作者信息

Jurberg Arnon D, Oliveira Aureo A de, Lenzi Henrique L, Coelho Paulo Marcos Z

机构信息

Laboratório de Patologia, Instituto Oswaldo Cruz-Fiocruz, Av. Augusto de Lima 1715, 30190-002 Belo Horizonte, MG, Brasil .

出版信息

Mem Inst Oswaldo Cruz. 2008 Feb;103(1):112-4. doi: 10.1590/s0074-02762008005000005. Epub 2008 Jan 31.

Abstract

It is still imperative to develop a parasitological technique highly sensitive for diagnosing schistosomiasis in epidemiological and individual surveys. A simple and cheap hatching device with a collecting container was manufactured and tested under experimental conditions. Twelve Kato-Katz slides were performed as golden standard for comparison. Quantitative results can be carried out by counting miracidia in a plate and parasite load can be calculated (miracidia/gram of feces). Statistically significant values were higher in the hatching test. More sensitive results, with statistical significance, were achieved using 1.5 g of feces (which corresponds to 36 Kato-Katz slides) than by using the Kato-Katz method. Advantages of this technique and its limitations are presented.

摘要

在流行病学和个体调查中,开发一种对血吸虫病诊断高度敏感的寄生虫学技术仍然至关重要。制造了一种带有收集容器的简单廉价孵化装置,并在实验条件下进行了测试。以12张加藤-卡茨涂片作为金标准进行比较。通过在平板中计数毛蚴可以获得定量结果,并可计算寄生虫负荷(每克粪便中的毛蚴数)。孵化试验中的统计学显著值更高。使用1.5克粪便(相当于36张加藤-卡茨涂片)比使用加藤-卡茨方法获得了更敏感且具有统计学意义的结果。本文介绍了该技术的优点及其局限性。

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