Stingu Catalina-Suzana, Eschrich Klaus, Rodloff Arne C, Schaumann Reiner, Jentsch Holger
Institute for Medical Microbiology and Epidemiology of Infectious Diseases, University of Leipzig, Liebigstr. 24, 04103 Leipzig, Germany.
Institute of Biochemistry, Medical Faculty, University of Leipzig, Johannisallee 30, 04103 Leipzig, Germany.
J Med Microbiol. 2008 Apr;57(Pt 4):495-499. doi: 10.1099/jmm.0.47649-0.
The aim of this study was to estimate differences in the prevalence of oral streptococcal species in the subgingival biofilm of patients with aggressive periodontitis and of healthy controls. Thirty-three patients with clinical and radiological proof of aggressive periodontitis and 20 healthy subjects were enrolled in this study. Clinical indices were recorded in a six-point measurement per tooth. Samples of the subgingival biofilm were taken with paper points from four teeth of each individual. Alpha- and non-haemolytic, small and catalase-negative colonies were biochemically identified using a rapid ID 32 STREP system and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A total of 118 strains of oral streptococci (11 species) were identified and Streptococcus sanguinis was found significantly more often in healthy subjects (P=0.001). Conversely, the absence of S. sanguinis was associated with high values of clinical indices (P=0.001-0.002). Aggressive periodontitis seems to be associated with a loss of colonization of S. sanguinis. Whether or not S. sanguinis offers protection against aggressive periodontitis needs to be determined. Otherwise, there were no significant differences in the distribution of oral streptococcal species in patients and healthy subjects.
本研究的目的是评估侵袭性牙周炎患者与健康对照者龈下生物膜中口腔链球菌种类的患病率差异。本研究纳入了33例有侵袭性牙周炎临床和影像学证据的患者以及20名健康受试者。对每颗牙齿进行六点测量记录临床指标。从每个个体的四颗牙齿上用纸尖采集龈下生物膜样本。使用快速ID 32 STREP系统和基质辅助激光解吸/电离飞行时间质谱对α溶血和非溶血、小菌落且过氧化氢酶阴性的菌落进行生化鉴定。共鉴定出118株口腔链球菌（11个种），在健康受试者中血链球菌的检出频率明显更高（P=0.001）。相反，血链球菌的缺失与临床指标的高值相关（P=0.001 - 0.002）。侵袭性牙周炎似乎与血链球菌的定植丧失有关。血链球菌是否能预防侵袭性牙周炎尚需确定。此外，患者和健康受试者口腔链球菌种类的分布没有显著差异。
