Identification and Correlation of and in Caries-active and Caries-free Children: A PCR Study.

AIM AND OBJECTIVES

Dental caries is currently considered an ecological imbalance within the oral biofilm leading to the dissolution of the tooth's hard tissues. It has been traditionally thought that two species belonging to the group, (SM) and (SS), are the etiologically responsible for the onset of dental decay.

MATERIALS AND METHODS

The present study was conducted on 40 children with caries-active (CA) and caries-free (CF). They were allocated into two groups, group I (CA) = 20 and group II (CF) = 20. The whole saliva was collected into the vials with buffer solution and was stored in cold storage. Polymerase chain reaction (PCR) was done to identify and correlate SM and SS in CA and CF children.

RESULTS

Comparison of mean SM level between CA and CF groups showed a statistically significant result at = 0.001. Spearman's correlation between caries score and SM showed a strong correlation of 0.77 between caries score and SM, which was statistically significant at = 0.001. Similarly, SS and caries scores showed a weak correlation of 0.22. Simple linear regression analysis to SM and caries score showed a significant increase of 4.74 units for 1 score increase in caries score, which is statistically significant.

CONCLUSION

The presence of SM levels in children with caries is significant, whereas, in CF children, SS levels are present in increased levels. A strong correlation was seen between caries scores and SM. The simple linear regression analysis predicts a statistically significant increase by 4.74 units per increase of 1 score of caries at < 0.001. As caries increase, SM count increases, but SS count decreases; as SS count increases, there is a reduction in SM counts.

HOW TO CITE THIS ARTICLE

Thimmegowda U, Belagatta V, Chikkanarasaiah N, Identification and Correlation of and in Caries-active and Caries-free Children: A PCR Study. Int J Clin Pediatr Dent 2023;16(1):9-15.