Liu Y, Janeway C A
Section of Immunobiology, Yale University School of Medicine, Howard Hughes Medical Institute, New Haven, Connecticut.
Adv Exp Med Biol. 1991;292:105-13. doi: 10.1007/978-1-4684-5943-2_12.
We have used anti-T cell mAbs as mimic ligands to study the effects of TCR/CD3 ligation of Th1 clones in the presence or absence of accessory cells. Our results demonstrated that ligation of TCR/CD3 in the presence of accessory cells induces proliferation of Th1 clone, while the same ligation in the absence of accessory cells results in death. This effect is inhibited by cyclosporin A and by anti-IFN-gamma mAbs and is restored by adding exogenous recombinant IFN-gamma tb CsA treated cells. We propose a model which could provide a general framework to explain activation, clonal anergy as well as clonal deletion of T lymphocytes during thymic development and in the peripheral.
我们已使用抗T细胞单克隆抗体作为模拟配体，以研究在有或无辅助细胞存在的情况下，TCR/CD3连接对Th1克隆的影响。我们的结果表明，在有辅助细胞存在的情况下，TCR/CD3的连接可诱导Th1克隆增殖，而在无辅助细胞存在的情况下进行相同的连接则导致细胞死亡。这种效应可被环孢素A和抗IFN-γ单克隆抗体抑制，并通过添加外源性重组IFN-γ使经CsA处理的细胞恢复。我们提出了一个模型，该模型可为解释胸腺发育期间及外周T淋巴细胞的激活、克隆无能以及克隆缺失提供一个通用框架。
