Zhao Jinfu, Bertoglio Bryan A, Gee Kyle R, Kay Alan R
Department of Biology, 336 BB, University of Iowa, Iowa City, IA 52242, USA.
Cell Calcium. 2008 Oct;44(4):422-6. doi: 10.1016/j.ceca.2008.01.006.
There has been some dispute in the literature as to the sensitivity of the zinc indicator FluoZin-3 to calcium, with suggestions that physiological levels of calcium and magnesium effectively occlude the response of the probe to zinc. In this communication we demonstrate that calcium concentrations as high as 10 mM do not prevent FluoZin-3 from detecting zinc elevations as low as 100 pM. Moreover, the inclusion of a few microM Ca-EDTA does not prevent FluoZin-3 from responding to increases in zinc concentration but does extend the dynamic range of the probe by reducing contaminating zinc levels and allowing the probe to respond to multiple zinc additions. In addition, we have derived a mathematical model to account for the kinetics of FluoZin-3 response to zinc in the presence of an additional zinc and calcium chelator.
关于锌指示剂FluoZin-3对钙的敏感性，文献中存在一些争议，有人认为生理水平的钙和镁会有效抑制该探针对锌的反应。在本通讯中，我们证明，高达10 mM的钙浓度不会阻止FluoZin-3检测低至100 pM的锌浓度升高。此外，加入几微摩尔的Ca-EDTA不会阻止FluoZin-3对锌浓度增加的反应，但确实通过降低污染锌水平并使探针能够对多次添加的锌做出反应来扩展了探针的动态范围。此外，我们推导了一个数学模型，以解释在存在额外的锌和钙螯合剂的情况下，FluoZin-3对锌反应的动力学。
