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高效液相色谱法分析基础化合物的挑战：提高分离的一些可能方法。

The challenges of the analysis of basic compounds by high performance liquid chromatography: some possible approaches for improved separations.

机构信息

Centre for Research in Biomedicine, University of the West of England, Frenchay, Bristol BS16 1QY, UK.

出版信息

J Chromatogr A. 2010 Feb 5;1217(6):858-80. doi: 10.1016/j.chroma.2009.11.068. Epub 2009 Dec 3.

DOI:10.1016/j.chroma.2009.11.068
PMID:20031138
Abstract

This review considers some of the difficulties encountered with the analysis of ionised bases using reversed-phase chromatography, such as detrimental interaction with column silanol groups, and overloading which both lead to poor peak shapes. Methods of overcoming these problems in reversed-phase (RP) separations, by judicious selection of the column and mobile phase conditions, are discussed. Hydrophilic interaction chromatography is considered as an alternative method for the separation of some basic compounds.

摘要

本文综述了反相色谱分析离子碱基时遇到的一些困难，如与柱硅羟基的有害相互作用以及过载，这两者都会导致峰形不佳。通过明智地选择柱和流动相条件，讨论了在反相（RP）分离中克服这些问题的方法。亲水相互作用色谱法被认为是分离某些碱性化合物的替代方法。

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