Rafat Mehrdad, Matsuura Takeshi, Li Fengfu, Griffith May
Department of Chemical Engineering, University of Ottawa, Ottawa, Ontario, Canada.
J Biomed Mater Res A. 2009 Mar 1;88(3):755-68. doi: 10.1002/jbm.a.31910.
Our objective was to develop collagen-based hydrogels as tissue substitutes for corneal transplantation. The design of the full-thickness corneal grafts includes prevention of cell migration onto the posterior surface of the implants, using a plasma-assisted surface modification technique. Briefly, the hydrogel materials were subjected to ammonia plasma functionalization followed by grafting of alginate macromolecules to the target surface. The treated materials surfaces showed observable decreases in endothelial cell attachment. The decrease in cell attachment and adhesion was dependant upon the concentration of alginate and plasma radio frequency (RF) power. High concentrations of alginate 5% (w/v) and high RF power of 100 W produced surfaces with minimal cell attachment. The plasma-alginate treatment did not adversely affect the optical or swelling properties of the constructs. Contact angle measurement analysis revealed that the posterior surface hydrophilicity significantly increased after the treatment. The grafting of alginate to the implants surfaces was confirmed by fourier transform infrared spectroscopy. Both of the untreated and alginate grafted corneal materials were found to be superior to human cornea in optical and swelling properties.
我们的目标是开发基于胶原蛋白的水凝胶作为角膜移植的组织替代物。全层角膜移植物的设计包括使用等离子体辅助表面改性技术防止细胞迁移到植入物的后表面。简而言之，水凝胶材料先进行氨等离子体功能化，然后将藻酸盐大分子接枝到目标表面。经处理的材料表面显示内皮细胞附着明显减少。细胞附着和黏附的减少取决于藻酸盐的浓度和等离子体射频（RF）功率。5%（w/v）的高浓度藻酸盐和100 W的高RF功率产生的表面细胞附着最少。等离子体-藻酸盐处理对构建体的光学或膨胀特性没有不利影响。接触角测量分析表明，处理后后表面亲水性显著增加。通过傅里叶变换红外光谱证实了藻酸盐接枝到植入物表面。未处理的和藻酸盐接枝的角膜材料在光学和膨胀特性方面均优于人角膜。
