Tazi A, Fajac I, Soler P, Valeyre D, Battesti J P, Hance A J
INSERM U 82, Faculté de Médecine Xavier Bichat, Paris, France.
Am Rev Respir Dis. 1991 Dec;144(6):1373-5. doi: 10.1164/ajrccm/144.6.1373.
gamma delta T-lymphocytes have been suggested to play a role in granuloma formation and to participate in the immune responses observed in tuberculosis and sarcoidosis. To further evaluate the role of gamma delta T-lymphocytes in these disorders, we used immunohistochemical techniques and specific monoclonal antibodies to characterize the number of distribution of T-lymphocytes expressing alpha beta and gamma delta T-cell receptors in granulomatous lesions from patients with sarcoidosis (n = 8) or tuberculosis (n = 4). The vast majority of T-lymphocytes present in lymph nodes from patients with tuberculosis or sarcoidosis expressed alpha beta TCR, whereas only rare gamma delta T-lymphocytes were observed inside or at the periphery of the granulomas. Recently formed granulomas induced by positive Kveim test reactions in patients with sarcoidosis also contained only small numbers of gamma delta T-lymphocytes, including those present in biopsies from two patients with increased numbers of circulating gamma delta T-lymphocytes. These observations demonstrate that T-cells involved in immune granulomatous reactions in sarcoidosis and tuberculosis express predominantly alpha beta TCR, and they do not support the conclusion that gamma delta T-lymphocytes necessarily play an important role in granuloma formation.
γδ T淋巴细胞被认为在肉芽肿形成中起作用，并参与在结核病和结节病中观察到的免疫反应。为了进一步评估γδ T淋巴细胞在这些疾病中的作用，我们使用免疫组织化学技术和特异性单克隆抗体来表征结节病患者（n = 8）或结核病患者（n = 4）肉芽肿病变中表达αβ和γδ T细胞受体的T淋巴细胞的分布数量。结核病或结节病患者淋巴结中存在的绝大多数T淋巴细胞表达αβ TCR，而在肉芽肿内部或周边仅观察到极少数γδ T淋巴细胞。结节病患者通过阳性Kveim试验反应诱导的新形成的肉芽肿也仅含有少量γδ T淋巴细胞，包括来自两名循环γδ T淋巴细胞数量增加的患者活检组织中的细胞。这些观察结果表明，参与结节病和结核病免疫肉芽肿反应的T细胞主要表达αβ TCR，并且不支持γδ T淋巴细胞必然在肉芽肿形成中起重要作用的结论。
