Müller M G, Georgakoudi I, Zhang Q, Wu J, Feld M S
Appl Opt. 2001 Sep 1;40(25):4633-46. doi: 10.1364/ao.40.004633.
The fluorescence from a turbid medium such as biologic tissue contains information about scattering and absorption, as well as the intrinsic fluorescence, i.e., the fluorescence from an optically thin sample of pure fluorophores. The interplay of scattering and absorption can result in severe distortion of the intrinsic spectral features. These distortions can be removed by use of a photon-migration-based picture and information from simultaneously acquired fluorescence and reflectance spectra. We present experimental evidence demonstrating the validity of such an approach for extracting the intrinsic fluorescence for a wide range of scatterer and absorber concentrations in tissue models, ex vivo and in vivo tissues. We show that variations in line shape and intensity in intrinsic tissue fluorescence are significantly reduced compared with the corresponding measured fluorescence.
来自诸如生物组织等混浊介质的荧光包含有关散射和吸收的信息，以及固有荧光，即来自纯荧光团光学薄样品的荧光。散射和吸收的相互作用会导致固有光谱特征的严重失真。通过使用基于光子迁移的图像以及同时获取的荧光和反射光谱信息，可以消除这些失真。我们提供了实验证据，证明了这种方法对于在组织模型、离体和体内组织中提取广泛散射体和吸收体浓度下的固有荧光的有效性。我们表明，与相应测量的荧光相比，固有组织荧光的线形和强度变化显著降低。
