光调制后失相成像（OPIOM）在恶劣光学条件下用于多重荧光成像。
Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.
机构信息
PASTEUR, Département de Chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS, Paris, France.
Laboratoire de Physique Théorique de la Matière Condensée (LPTMC), Sorbonne Université, Centre National de la Recherche Scientifique (CNRS), Paris, Cedex 05, France.
出版信息
Methods Mol Biol. 2021;2350:191-227. doi: 10.1007/978-1-0716-1593-5_13.
Fluorescence imaging has become a powerful tool for observations in biology. Yet it has also encountered limitations to overcome optical interferences of ambient light, autofluorescence, and spectrally interfering fluorophores. In this account, we first examine the current approaches which address these limitations. Then we more specifically report on Out-of-Phase Imaging after Optical Modulation (OPIOM), which has proved attractive for highly selective multiplexed fluorescence imaging even under adverse optical conditions. After exposing the OPIOM principle, we detail the protocols for successful OPIOM implementation.
荧光成像是生物学观察的有力工具。然而，它也遇到了需要克服环境光干扰、自发荧光和光谱干扰荧光团的限制。在本报告中，我们首先检查了目前解决这些限制的方法。然后我们更具体地报告了光调制后离相成像（OPIOM），即使在不利的光学条件下，它也被证明对高度选择性的多路复用荧光成像很有吸引力。在介绍了 OPIOM 原理之后，我们详细说明了成功实施 OPIOM 的方案。
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