梅毒的血清学诊断：采用酶联免疫吸附测定法检测针对各个重组梅毒螺旋体抗原的抗体。
Serological diagnosis of syphilis: enzyme-linked immunosorbent assay to measure antibodies to individual recombinant Treponema pallidum antigens.
作者信息
Martin Irene E, Lau Allan, Sawatzky Pam, Tsang Raymond S W, Cuff Wilfred, Lee Craig, Macpherson Paul A, Mazzulli Tony
机构信息
Pathogenic Neisseria and Syphilis Diagnostic Section, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
出版信息
J Immunoassay Immunochem. 2008;29(2):143-51. doi: 10.1080/15321810801887771.
We standardized an indirect ELISA for measurement of serum antibody levels to four individual treponemal recombinant proteins that have been commonly used in a number of commercial EIAs, mostly as a mixture of antigens. When tested with 127 syphilis-negative and 37 secondary syphilis sera, ELISA O.D.s obtained for each of the four antigens clearly distinguished between these two groups of samples. Sensitivity and specificity of 100% was obtained with the current set of samples. Further evaluations with sera from different stages of syphilis can help to define the applications of this ELISA test for each of the four antigens studied.
我们标准化了一种间接酶联免疫吸附测定法（ELISA），用于检测血清中针对四种梅毒螺旋体重组蛋白的抗体水平。这四种重组蛋白在许多商业酶联免疫吸附测定法中常用，大多作为抗原混合物使用。用127份梅毒阴性血清和37份二期梅毒血清进行检测时，四种抗原各自的ELISA光密度值能清楚地区分这两组样本。对当前这组样本而言，灵敏度和特异性均达到了100%。使用来自梅毒不同阶段的血清进行进一步评估，有助于明确该ELISA检测法对所研究的四种抗原各自的应用情况。
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