Schmidt B L, Edjlalipour M, Luger A
Ludwig Boltzmann Institute of Dermato-Venerological Serodiagnostics, Department of Dermatology, Hospital of the City of Vienna, Lainz, A-1130 Vienna, Austria.
J Clin Microbiol. 2000 Mar;38(3):1279-82. doi: 10.1128/JCM.38.3.1279-1282.2000.
Nine different enzyme-linked immunosorbent assays (ELISAs) with a sonicate or recombinant proteins of Treponema pallidum as antigen have been evaluated comparatively by testing 52 highly selected sera from patients with primary syphilis, all negative in the microhemagglutination test for T. pallidum (MHA-TP). Eight tests exhibited greater sensitivity (48.5 to 76.9%) than the commonly used Venereal Disease Research Laboratory test (44.2%). Higher sensitivity could be related to (i) the volume and dilution of the serum, (ii) the design of the assay (capture and competitive tests showed higher sensitivity than sandwich-based assays), and (iii) the ability to detected specific immunoglobulin M antibodies. The specificity of the ICE Syphilis and the Enzygnost Syphilis tests was 99.5 and 99.8%, respectively, as determined by routine testing of 2, 053 unselected sera in comparison with the MHA-TP test. ELISAs tested offered high sensitivity in patients with primary syphilis; however, recommendations to use these tests as screening assays do need further data on specificity and reactivity in late stages of the disease.
通过检测52份从一期梅毒患者中高度筛选出的血清（所有血清在梅毒螺旋体微量血凝试验（MHA - TP）中均为阴性），对九种以梅毒螺旋体超声裂解物或重组蛋白作为抗原的不同酶联免疫吸附测定（ELISA）进行了比较评估。八项检测表现出比常用的性病研究实验室试验（44.2%）更高的敏感性（48.5%至76.9%）。更高的敏感性可能与以下因素有关：（i）血清的体积和稀释度；（ii）检测方法的设计（捕获法和竞争法检测表现出比基于夹心的检测方法更高的敏感性）；以及（iii）检测特异性免疫球蛋白M抗体的能力。通过与MHA - TP试验比较，对2053份未筛选血清进行常规检测，确定ICE梅毒检测和Enzygnost梅毒检测的特异性分别为99.5%和99.8%。所测试的ELISA在一期梅毒患者中具有高敏感性；然而，要将这些检测用作筛查试验，还需要关于疾病后期特异性和反应性的进一步数据。
