Brinkman Mary Beth, McKevitt Matthew, McLoughlin Melanie, Perez Carla, Howell Jerrilyn, Weinstock George M, Norris Steven J, Palzkill Timothy
Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
J Clin Microbiol. 2006 Mar;44(3):888-91. doi: 10.1128/JCM.44.3.888-891.2006.
To identify antigens important in the human immune response to syphilis, the serum antibody reactivity of syphilitic patients was examined with 908 of the 1,039 proteins in the proteome of Treponema pallidum subsp. pallidum using a protein array enzyme-linked immunosorbent assay. Thirty-four proteins exhibited significant reactivity when assayed with human sera from patients in the early latent stage of syphilis. A subset of antigens identified were further scrutinized for antibody reactivity at primary, secondary, and latent disease stages, and the results demonstrate that the humoral immune response to individual T. pallidum proteins develops at different rates during the time course of infection.
为了鉴定在人类梅毒免疫反应中重要的抗原，使用蛋白质阵列酶联免疫吸附测定法，检测了梅毒患者的血清抗体与梅毒螺旋体苍白亚种蛋白质组中1039种蛋白质中的908种的反应性。用梅毒早期潜伏阶段患者的人血清进行检测时，有34种蛋白质表现出显著反应性。对鉴定出的一部分抗原在一期、二期和潜伏疾病阶段的抗体反应性进行了进一步仔细检查，结果表明，在感染过程中，针对梅毒螺旋体单个蛋白质的体液免疫反应以不同速率发展。
