Alves C M, Yang Y, Marton D, Carnes D L, Ong J L, Sylvia V L, Dean D D, Reis R L, Agrawal C M
Department of Polymer Engineering, 3B's Research Group-Biomaterials, Biodegradables, and Biomimetics, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal.
J Biomed Mater Res B Appl Biomater. 2008 Oct;87(1):59-66. doi: 10.1002/jbm.b.31068.
We have studied the influence of oxygen radio frequency glow discharge (RfGD) on the surface and bulk properties of poly(D,L-lactic acid) (PDLLA) and the effect of this surface modification on both protein adsorption and bone cell behavior. PDLLA films were characterized before and after plasma surface modification by water contact angle, surface energy, and adhesion tension of water as well as by scanning electron microscopy (SEM), X-ray electron spectroscopy (XPS), and Fourier transform infra-red (FTIR) spectroscopy. RfGD-films showed an increase in hydrophilicity and surface energy when compared with untreated films. Surface morphological changes were observed by SEM. Chemical analysis indicated significant differences in both atomic percentages and oxygen functional group. Protein adsorption was evaluated by combining solute depletion and spectroscopic techniques. Bovine serum albumin (BSA), fibronectin (FN), vitronectin (VN), and fetal bovine serum (FBS) were used in this study. RfGD-treated surfaces adsorbed more BSA and FN from single specie solutions than FBS that is a more complex, multi-specie solution. MG63 osteoblast-like cells and primary cultures of fetal rat calvarial (FRC) cells were used to assess both the effect of RfGD treatment and protein adsorption on cell attachment and proliferation. In the absence of preadsorbed proteins, cells could not distinguish between treated and untreated surfaces, with the exception of MG63 cells cultured for longer periods of time. In contrast, the adsorption of proteins increased the cells' preference for treated surfaces, thus indicating a crucial role for adsorbed proteins in mediating the response of osteogenic cells to the RfGD-treated PDLLA surface.
我们研究了氧射频辉光放电（RfGD）对聚（D，L-乳酸）（PDLLA）表面和本体性能的影响，以及这种表面改性对蛋白质吸附和骨细胞行为的作用。通过水接触角、表面能、水的粘附张力以及扫描电子显微镜（SEM）、X射线电子能谱（XPS）和傅里叶变换红外（FTIR）光谱对等离子体表面改性前后的PDLLA薄膜进行了表征。与未处理的薄膜相比，RfGD处理的薄膜亲水性和表面能增加。通过SEM观察到表面形态变化。化学分析表明原子百分比和氧官能团均存在显著差异。通过结合溶质耗尽和光谱技术评估蛋白质吸附。本研究使用了牛血清白蛋白（BSA）、纤连蛋白（FN）、玻连蛋白（VN）和胎牛血清（FBS）。RfGD处理的表面从单一组分溶液中吸附的BSA和FN比FBS（一种更复杂的多组分溶液）更多。使用MG63成骨样细胞和胎鼠颅骨（FRC）细胞原代培养物来评估RfGD处理和蛋白质吸附对细胞附着和增殖的影响。在没有预吸附蛋白质的情况下，细胞无法区分处理过的表面和未处理的表面，但长时间培养的MG63细胞除外。相比之下，蛋白质的吸附增加了细胞对处理过表面的偏好，从而表明吸附的蛋白质在介导成骨细胞对RfGD处理的PDLLA表面的反应中起关键作用。
