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猪血清中检测奥杰斯基病毒抗体的中和试验与间接荧光抗体技术的比较研究

A comparative study of the neutralisation test and the indirect fluorescent antibody technique for the detection of antibodies to the virus of Aujeszky in pig sera.

作者信息

Wirahadiredja R M, Rondhuis P R

出版信息

Tijdschr Diergeneeskd. 1976 Oct 15;101(20):1125-8.

PMID:189453
Abstract

A microneutralisation test and an indirect fluorescent antibody technique for the detection of antibodies to Aujeszky-virus in pig sera are described. All the sera were tested with both techniques and the results were compared. Serum dilutions of less than 1:32 which produced a positive reaction in the indirect FAT were considered nonspecific. Bij contrast, all neutralising antibody titers of less than 1:8 gave nonspecific reactions in the indirect FAT.

摘要

本文描述了用于检测猪血清中奥耶斯基病毒抗体的微量中和试验和间接荧光抗体技术。所有血清都用这两种技术进行了检测,并对结果进行了比较。间接荧光抗体试验中产生阳性反应的血清稀释度小于1:32的被认为是非特异性的。相比之下,间接荧光抗体试验中所有中和抗体效价小于1:8的都产生非特异性反应。

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