Schauer Nicolas, Semel Yaniv, Balbo Ilse, Steinfath Matthias, Repsilber Dirk, Selbig Joachim, Pleban Tzili, Zamir Dani, Fernie Alisdair R
Max-Planck Institute for Molecular Plant Physiology, 14476 Potsdam-Golm, Germany.
Plant Cell. 2008 Mar;20(3):509-23. doi: 10.1105/tpc.107.056523. Epub 2008 Mar 25.
To evaluate components of fruit metabolic composition, we have previously metabolically phenotyped tomato (Solanum lycopersicum) introgression lines containing segmental substitutions of wild species chromosome in the genetic background of a cultivated variety. Here, we studied the hereditability of the fruit metabolome by analyzing an additional year's harvest and evaluating the metabolite profiles of lines heterozygous for the introgression (ILHs), allowing the evaluation of putative quantitative trait locus (QTL) mode of inheritance. These studies revealed that most of the metabolic QTL (174 of 332) were dominantly inherited, with relatively high proportions of additively (61 of 332) or recessively (80 of 332) inherited QTL and a negligible number displaying the characteristics of overdominant inheritance. Comparison of the mode of inheritance of QTL revealed that several metabolite pairs displayed a similar mode of inheritance of QTL at the same chromosomal loci. Evaluation of the association between morphological and metabolic traits in the ILHs revealed that this correlation was far less prominent, due to a reduced variance in the harvest index within this population. These data are discussed in the context of genomics-assisted breeding for crop improvement, with particular focus on the exploitation of wide biodiversity.
为了评估果实代谢成分的组成部分，我们之前对番茄（Solanum lycopersicum）渗入系进行了代谢表型分析，这些渗入系在栽培品种的遗传背景中包含野生种染色体的片段性替换。在这里，我们通过分析额外一年的收获物并评估渗入杂合系（ILHs）的代谢物谱来研究果实代谢组的遗传性，从而能够评估假定的数量性状位点（QTL）的遗传模式。这些研究表明，大多数代谢QTL（332个中的174个）呈显性遗传，具有相对较高比例的加性遗传（332个中的61个）或隐性遗传（332个中的80个）QTL，而表现出超显性遗传特征的QTL数量可忽略不计。QTL遗传模式的比较表明，几对代谢物在相同染色体位点显示出相似的QTL遗传模式。对ILHs中形态和代谢性状之间关联的评估表明，由于该群体中收获指数的方差减小，这种相关性远不那么显著。我们在作物改良的基因组辅助育种背景下讨论了这些数据，特别关注广泛生物多样性的利用。
