Kulak M V, Netesova N A, Belavin P A, Seregina E V, Ignat'ev G M
Mol Gen Mikrobiol Virusol. 2008(1):34-7.
Recombinant proteins rN (nucleocapsid) and rH/Nh (hemagglutinin) of the measles virus strain NovO/96 of genotype A were obtained. The immunobiological properties of the proteins were studied in the reaction with a panel of positive and negative sera. Mice of the line Balb/C were immunized with recombinant proteins and native antigen of the measles virus strain NovO/96 in order to obtain hyperimmune serum and its analysis using ELISA (enzyme-linked immunosorbent assay) and PRN (plaque reduction neutralization). The hyperimmune serum against recombinant proteins and native antigen of the measles virus strain NovO/96 were found to be highly active in ELISA. The antibodies against the proteins rN and rH/Nh were found to be able to neutralize the virus in titer 1:13.5 and 1:22.9, respectively. The neutralization titer of the antibodies generated against native antigen of the measles virus strain NovO/96 was 1:25.7.
获得了基因型A的麻疹病毒株NovO/96的重组蛋白rN（核衣壳蛋白）和rH/Nh（血凝素）。在与一组阳性和阴性血清的反应中研究了这些蛋白的免疫生物学特性。用重组蛋白和麻疹病毒株NovO/96的天然抗原免疫Balb/C系小鼠，以获得超免疫血清，并使用酶联免疫吸附测定（ELISA）和蚀斑减少中和试验（PRN）对其进行分析。发现针对麻疹病毒株NovO/96重组蛋白和天然抗原的超免疫血清在ELISA中具有高活性。发现针对蛋白rN和rH/Nh的抗体能够分别以1:13.5和1:22.9的效价中和病毒。针对麻疹病毒株NovO/96天然抗原产生的抗体的中和效价为1:25.7。
