Wu Yuanyuan, Liu Ying, Chesnut Jonathan D, Rao Mahendra S
Department of Human Genetics, University of Utah, Salt Lake City, UT, USA.
Methods Mol Biol. 2008;438:39-53. doi: 10.1007/978-1-59745-133-8_5.
Isolation and characterization of neural stem cells and lineage-specific progenitors provide important information for central nervous system development study and regenerative medicine. We describe methods for dissection of rodent embryonic spinal cords by enzymatic separation, and isolation and enrichment (or purification) of neuronal and glial precursors at different developing stages by fluorescence-activated cell sorting.
神经干细胞和谱系特异性祖细胞的分离与鉴定为中枢神经系统发育研究和再生医学提供了重要信息。我们描述了通过酶解分离啮齿动物胚胎脊髓的方法，以及通过荧光激活细胞分选在不同发育阶段分离和富集（或纯化）神经元和胶质前体细胞的方法。
