Kim Kitai, Ng Kitwa, Rugg-Gunn Peter J, Shieh Jae-Hung, Kirak Oktay, Jaenisch Rudolf, Wakayama Teruhiko, Moore Malcolm A, Pedersen Roger A, Daley George Q
Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, MA 02115, USA.
Cell Stem Cell. 2007 Sep 13;1(3):346-52. doi: 10.1016/j.stem.2007.07.001. Epub 2007 Aug 2.
Parthenogenesis and somatic cell nuclear transfer (SCNT) are two methods for deriving embryonic stem (ES) cells that are genetically matched to the oocyte donor or somatic cell donor, respectively. Using genome-wide single nucleotide polymorphism (SNP) analysis, we demonstrate distinct signatures of genetic recombination that distinguish parthenogenetic ES cells from those generated by SCNT. We applied SNP analysis to the human ES cell line SCNT-hES-1, previously claimed to have been derived by SCNT, and present evidence that it represents a human parthenogenetic ES cell line. Genome-wide SNP analysis represents a means to validate the genetic provenance of an ES cell line.
孤雌生殖和体细胞核移植（SCNT）是分别用于获得与卵母细胞供体或体细胞供体基因匹配的胚胎干细胞（ES细胞）的两种方法。利用全基因组单核苷酸多态性（SNP）分析，我们证明了遗传重组的独特特征，这些特征将孤雌生殖的ES细胞与通过SCNT产生的ES细胞区分开来。我们将SNP分析应用于之前声称通过SCNT获得的人ES细胞系SCNT-hES-1，并提供证据表明它代表一个人孤雌生殖的ES细胞系。全基因组SNP分析是验证ES细胞系遗传来源的一种手段。
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