Kim Kitai, Lerou Paul, Yabuuchi Akiko, Lengerke Claudia, Ng Kitwa, West Jason, Kirby Andrew, Daly Mark J, Daley George Q
Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA.
Science. 2007 Jan 26;315(5811):482-6. doi: 10.1126/science.1133542. Epub 2006 Dec 14.
Genetically matched pluripotent embryonic stem (ES) cells generated via nuclear transfer or parthenogenesis (pES cells) are a potential source of histocompatible cells and tissues for transplantation. After parthenogenetic activation of murine oocytes and interruption of meiosis I or II, we isolated and genotyped pES cells and characterized those that carried the full complement of major histocompatibility complex (MHC) antigens of the oocyte donor. Differentiated tissues from these pES cells engrafted in immunocompetent MHC-matched mouse recipients, demonstrating that selected pES cells can serve as a source of histocompatible tissues for transplantation.
通过核移植或孤雌生殖产生的基因匹配多能胚胎干细胞（pES细胞）是用于移植的组织相容性细胞和组织的潜在来源。在对小鼠卵母细胞进行孤雌激活并中断减数分裂I或II后，我们分离了pES细胞并进行基因分型，对那些携带卵母细胞供体主要组织相容性复合体（MHC）抗原完整互补体的细胞进行了表征。这些pES细胞分化的组织移植到具有免疫活性的MHC匹配小鼠受体中，表明所选的pES细胞可作为移植用组织相容性组织的来源。
