Balajee S Arunmozhi, de Valk Hanneke A, Lasker Brent A, Meis Jacques F G M, Klaassen Corné H W
Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States.
J Microbiol Methods. 2008 Jun;73(3):252-6. doi: 10.1016/j.mimet.2008.02.011. Epub 2008 Feb 23.
A microsatellite assay based on short tandem repeats (STRAf) has been recently described as a discriminatory, high throughput assay for fingerprinting Aspergillus fumigatus isolates. However, the STRAf assay has not been tested for its utility in outbreak settings where it is critical to distinguish clonal clusters from genetically unrelated genotypes. In the present study, employing a panel of epidemiologically linked A. fumigatus isolates obtained from 6 different outbreaks of invasive aspergillosis (IA), we demonstrate that the STRAf assay can be a valuable molecular tool to support epidemiological investigations. We also report for the first time the detection of microvariation events in the A. fumigatus population studied.
一种基于短串联重复序列的微卫星分析方法（STRAf）最近被描述为一种用于烟曲霉分离株指纹识别的鉴别性高通量分析方法。然而，STRAf分析方法尚未在暴发环境中进行实用性测试，而在这种环境中，区分克隆簇与遗传无关的基因型至关重要。在本研究中，我们使用一组从6起不同的侵袭性曲霉病（IA）暴发中获得的具有流行病学关联的烟曲霉分离株，证明STRAf分析方法可以成为支持流行病学调查的有价值的分子工具。我们还首次报告了在所研究的烟曲霉群体中检测到微变异事件。
