Pontes Laís, Perini Leme Giordano Ana Luisa, Reichert-Lima Franqueline, Gualtieri Beraquet Caio Augusto, Leite Pigolli Guilherme, Arai Teppei, Ribeiro José Dirceu, Gonçalves Aline Cristina, Watanabe Akira, Goldman Gustavo Henrique, Moretti Maria Luiza, Zaninelli Schreiber Angélica
Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas 13083-887, Brazil.
Division of Clinical Research, Medical Mycology Research Center, Chiba University, Chiba 260-0856, Japan.
J Fungi (Basel). 2024 Jun 29;10(7):461. doi: 10.3390/jof10070461.
Approximately 60% of individuals with cystic fibrosis (CF) are affected by infection. This condition is correlated with a decline in lung function and is identified as an independent risk factor contributing to hospital admissions among CF patients. This study investigates the dynamic interplay of within the context of CF patients, tracing its evolution over time, with a specific emphasis on colonization dynamics.
An analysis was conducted on 83 sequential isolates derived from sputum samples of six patients receiving care at a renowned CF hospital in Brazil. Employing microsatellite genotyping techniques, alongside an investigation into 51A gene mutations, this research sheds light on the genetic variations, colonization, and resistance of within the CF respiratory environment.
Our research findings indicate that CF patients can harbor strains from the same clonal complexes for prolonged periods. Additionally, we identified that clinical isolates have the potential to spread among patients in the same healthcare facility, evidencing hospital contamination. Two patients who underwent long-term Itraconazole treatment did not show phenotypic resistance. However, one of these patients exhibited mutations in the 51A gene, indicating the need to monitor resistance to azoles in these patients colonized for long periods by . We also observed co-colonization or co-infection involving multiple genotypes in all patients over time.
This comprehensive examination offers valuable insights into the pathogenesis of infections in CF patients, potentially shaping future therapeutic strategies and management approaches. This enhanced understanding contributes to our knowledge of impact on disease progression in individuals with cystic fibrosis. Additionally, the study provides evidence of cross-contamination among patients undergoing treatment at the same hospital.
约60%的囊性纤维化（CF）患者受到感染影响。这种情况与肺功能下降相关，并被确定为导致CF患者住院的独立危险因素。本研究在CF患者的背景下调查了[具体病原体未明确]的动态相互作用，追踪其随时间的演变，特别强调定植动态。
对来自巴西一家著名CF医院接受治疗的6名患者痰液样本中的83株连续[具体病原体未明确]分离株进行了分析。采用微卫星基因分型技术，同时对51A基因突变进行调查，本研究揭示了CF呼吸道环境中[具体病原体未明确]的基因变异、定植和耐药情况。
我们的研究结果表明，CF患者可长期携带来自同一克隆复合体的[具体病原体未明确]菌株。此外，我们发现临床分离株有可能在同一医疗机构的患者之间传播，证明存在医院污染。两名接受长期伊曲康唑治疗的患者未表现出表型耐药。然而，其中一名患者在51A基因中出现了突变，表明需要监测这些长期被[具体病原体未明确]定植的患者对唑类药物的耐药性。随着时间的推移，我们还观察到所有患者都存在涉及多种基因型的共定植或共感染。
这项全面的检查为CF患者[具体病原体未明确]感染的发病机制提供了有价值的见解，可能会塑造未来的治疗策略和管理方法。这种深入的理解有助于我们了解[具体病原体未明确]对囊性纤维化患者疾病进展的影响。此外，该研究提供了同一医院接受治疗的患者之间交叉污染的证据。
