Arana-Gabriel Yolanda, Burrola-Aguilar Cristina, Garibay-Orijel Roberto, Matías-Ferrer Noemí, Franco-Maass Sergio, Mata Gerardo
Universidad Autónoma del Estado de México, Facultad de Ciencias, Centro de Investigación en Recursos Bióticos, Toluca, Mexico.
Universidad Autónoma del Estado de México, Facultad de Ciencias, Centro de Investigación en Recursos Bióticos, Toluca, Mexico.
Braz J Microbiol. 2018 Jul-Sep;49(3):632-640. doi: 10.1016/j.bjm.2017.12.002. Epub 2018 Feb 10.
The present study conducted a genetic characterization and determined growth rate and biomass production in solid and liquid media, using strains obtained from wild edible sporomes of Lyophyllum that grow in high mountains. Vegetative isolation was used to obtain a total of four strains, which were divided into two clades within the section Difformia: Lyophyllum sp. and Lyophyllum aff. shimeji. Growth rate and biomass production were influenced by both the culture media and the strains. In a potato dextrose agar medium, the strains presented a higher growth rate, while in a malt extract-peptone and yeast agar medium, the growth rate was lower, but with a higher biomass production that was equal to that in the malt extract-peptone and yeast liquid medium.
本研究对从生长在高山上的野生可食用裂褶菌子实体中获得的菌株进行了遗传特征分析，并测定了其在固体和液体培养基中的生长速率及生物量产量。采用营养分离法共获得4个菌株，这些菌株在异形组内分为两个进化枝：裂褶菌属和近白灵侧耳裂褶菌。生长速率和生物量产量受培养基和菌株的影响。在马铃薯葡萄糖琼脂培养基中，菌株呈现出较高的生长速率，而在麦芽提取物 - 蛋白胨和酵母琼脂培养基中，生长速率较低，但生物量产量较高，且与麦芽提取物 - 蛋白胨和酵母液体培养基中的生物量产量相当。
