血红素蛋白的低温辐射分解还原：最大化剂量依赖性产率。
Cryoradiolytic reduction of heme proteins: Maximizing dose dependent yield.
作者信息
Denisov Ilia G, Victoria Doreen C, Sligar Stephen G
机构信息
University of Illinois Urbana-Champaign, Department of Biochemistry, Urbana IL, 61801.
出版信息
Radiat Phys Chem Oxf Engl 1993. 2007 Apr;76(4):714-721. doi: 10.1016/j.radphyschem.2006.03.002.
Abstract
Radiolytic reduction in frozen solutions and crystals is a useful method for generation of trapped intermediates in protein based radical reactions. In this communication we define the conditions which provide the maximum yield of one electron reduced myoglobin at 77 K using (60)Co γ-irradiation in aqueous glycerol glass. The yield reached 50% after 20 kGy, was almost complete at ∼160 kGy total dose, and does not depend on the protein concentration in the range 0.01 - 5 mM.
摘要
冷冻溶液和晶体中的辐射分解还原是在基于蛋白质的自由基反应中生成捕获中间体的一种有用方法。在本通讯中，我们定义了在77K下使用（60）Coγ射线辐照水甘油玻璃时，产生单电子还原肌红蛋白最大产率的条件。在20kGy后产率达到50%，在总剂量约160kGy时几乎完全，并且在0.01 - 5mM范围内不依赖于蛋白质浓度。
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