脑脊液中疱疹病毒DNA的检测及其与临床症状的相关性。
Detection of herpesvirus DNA in cerebrospinal fluid and correlation with clinical symptoms.
作者信息
Plentz A, Jilg W, Kochanowski B, Ibach B, Knöll A
机构信息
Institute of Medical Microbiology and Hygiene, University of Regensburg, F-J-Strauss-Allee 11, 93053, Regensburg, Germany.
出版信息
Infection. 2008 Mar;36(2):158-62. doi: 10.1007/s15010-007-6354-y. Epub 2008 Mar 31.
BACKGROUND
Novel PCR techniques can detect minute quantities of herpesvirus DNA in cerebrospinal fluid (CSF). The clinical significance of such findings is not always clear.
PATIENTS AND METHODS
(a) Investigation of clinical characteristics of 76 patients with herpesvirus DNA detection in CSF. (b) Screening for herpesvirus DNA in CSF samples of 208 patients without clinical signs of herpesvirus infection.
RESULTS
(a) Eleven of 76 herpesvirus-DNA-positive patients did not show symptoms usually associated with the detected virus (HSV-1/2, n = 5; EBV, n = 6). (b) Two of 208 patients without hint for herpesvirus infection had HHV-6 DNA of low concentration in CSF.
CONCLUSIONS
The detection of low-level herpesvirus replication in CSF by highly sensitive PCR assays requires critical evaluation.
背景
新型聚合酶链反应（PCR）技术能够检测脑脊液（CSF）中微量的疱疹病毒DNA。此类检测结果的临床意义并不总是明确的。
患者与方法
（a）对76例脑脊液中检测出疱疹病毒DNA的患者的临床特征进行调查。（b）对208例无疱疹病毒感染临床症状患者的脑脊液样本进行疱疹病毒DNA筛查。
结果
（a）76例疱疹病毒DNA阳性患者中有11例未表现出通常与所检测病毒相关的症状（单纯疱疹病毒1型/2型，5例；EB病毒，6例）。（b）208例无疱疹病毒感染迹象的患者中有2例脑脊液中存在低浓度的人疱疹病毒6型DNA。
结论
通过高灵敏度PCR检测法在脑脊液中检测到低水平疱疹病毒复制需要进行严谨评估。
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