禽肉瘤病毒聚合酶将RNA高效转录为DNA。

Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase.

作者信息

Taylor J M, Illmensee R, Summers J

出版信息

Biochim Biophys Acta. 1976 Sep 6;442(3):324-30. doi: 10.1016/0005-2787(76)90307-5.

Abstract

The DNAase digestion end-product of calf thymus DNA contains oligonucleotides that will function as primers for the efficient transcription into DNA of many naturally-occurring RNA's by purified avian sarcoma virus RNA-directed DNA polymerase. The labeled DNA transcripts so obtained are valuable as probes for molecular hybridization studies. Typical applications of the method include the efficient transcription into DNA of 18 and 28 S rRNA as well as the RNA's of avian sarcoma virus, polio virus, influenza virus, satellite tobacco necrosis virus and tobacco mosaic virus. In addition, when these primers are added to avian sarcoma virus particles that have been partially-disrupted with non-ionic detergent there is 6-fold stimulation of the endogenous RNA-directed DNA synthesis.

摘要

小牛胸腺DNA的脱氧核糖核酸酶消化终产物含有寡核苷酸，这些寡核苷酸可作为引物，用于通过纯化的禽肉瘤病毒RNA指导的DNA聚合酶将许多天然存在的RNA高效转录为DNA。如此获得的标记DNA转录本作为分子杂交研究的探针很有价值。该方法的典型应用包括将18和28 S rRNA以及禽肉瘤病毒、脊髓灰质炎病毒、流感病毒、卫星烟草坏死病毒和烟草花叶病毒的RNA高效转录为DNA。此外，当将这些引物添加到已用非离子去污剂部分破坏的禽肉瘤病毒颗粒中时，内源性RNA指导的DNA合成会受到6倍的刺激。