禽成髓细胞瘤病毒RNA指导的DNA聚合酶合成辛德毕斯病毒互补DNA。

Synthesis of Sindbis virus complementary DNA by avian myeloblastosis virus RNA-directed DNA polymerase.

作者信息

Yuferov V, Grandgenett D P, Bondurant M, Riggin C, Tigges M

出版信息

Biochim Biophys Acta. 1978 Jul 24;519(2):348-55. doi: 10.1016/0005-2787(78)90087-4.

Abstract

Sindbis virus 42 S RNA was efficiently transcribed into complementary DNA (CDNA) by avian myeloblastosis virus alphabeta DNA polymerase using oligo- (dT) or single-stranded calf thymus DNA as primers. Both of the Sindbis virus cDNA products were able to protect 60% of 125I-labeled Sindbis virus RNA, at near equal weight ratios, from RNAase A and T1 digestion. Using hybridization kinetics, the Crt 1/2 value for hybridization of the calf thymus-primed cDNA product with excess Sindbis RNA was determined to be 1.8 9 10-2 mol . s . 1-1. Thes data demonstrate that the Sindbis virus cDNA products are relatively uniform representations of Sindbis virus RNA sequences.

摘要

辛德毕斯病毒42S RNA通过禽成髓细胞瘤病毒αβ DNA聚合酶，以寡聚（dT）或单链小牛胸腺DNA为引物，被高效转录成互补DNA（cDNA）。两种辛德毕斯病毒cDNA产物能够以近乎相等的重量比，保护60%的125I标记的辛德毕斯病毒RNA不被核糖核酸酶A和T1消化。利用杂交动力学，测定小牛胸腺引物cDNA产物与过量辛德毕斯RNA杂交的Crt 1/2值为1.8×10-2 mol·s·1-1。这些数据表明，辛德毕斯病毒cDNA产物是辛德毕斯病毒RNA序列的相对均匀的代表。