Taylor J M, Hsu T W, Lai M M
J Virol. 1978 May;26(2):479-84. doi: 10.1128/JVI.26.2.479-484.1978.
Full-size single-stranded DNA transcripts of the avian RNA tumor virus genome were isolated from the products of the endogenous reaction of detergent-disrupted avian sarcoma virus particles. These transcripts were converted with Escherichia coli DNA polymerase I and 32P-labeled nucleoside triphosphates into labeled double-stranded DNA. The latter DNA was used to map the sites of action of seven restriction enzymes (Pvu I, Hpa I, Kpn I, Xba I, EcoRI, HindIII, and Xho I) on the genome of three strains of avian sarcoma virus (Prague B, Prague C, and Bratislava 77).
从去污剂裂解的禽肉瘤病毒颗粒的内源性反应产物中分离出禽RNA肿瘤病毒基因组的全长单链DNA转录本。这些转录本用大肠杆菌DNA聚合酶I和32P标记的核苷三磷酸转化为标记的双链DNA。后者的DNA用于绘制七种限制性内切酶（Pvu I、Hpa I、Kpn I、Xba I、EcoRI、HindIII和Xho I）在三株禽肉瘤病毒（布拉格B、布拉格C和布拉迪斯拉发77）基因组上的作用位点。
