• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

未整合的线性脾坏死病毒DNA中的核糖核苷酸。

Ribonucleotides in unintegrated linear spleen necrosis virus DNA.

作者信息

Chen I S, Temin H M

出版信息

J Virol. 1980 Mar;33(3):1058-73. doi: 10.1128/JVI.33.3.1058-1073.1980.

DOI:10.1128/JVI.33.3.1058-1073.1980
PMID:7365874
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC288639/
Abstract

The structure of unintegrated spleen necrosis virus DNA was characterized by using various chemical and enzymatic treatments in conjunction with denaturing gels and nucleic acid hybridization probes. Throughout the course of the viral infection, the predominant species of viral DNA was that of a linear double-stranded molecule containing ribonucleotides covalently joined to the DNA. The majority of both - and + strands were continuous. The ribonucleotide linkages appeared to be relatively short, and the base composition and distribution of the ribonucleotide linkages were heterogeneous. On the average, the - strand had fewer of the ribonucleotide linkages than did the strand. Viral DNA containing ribonucleotide linkages was infectious in DNA transfection assays. The structure of spleen necrosis virus DNA was different from that of Schmidt-Ruppin Rous sarcoma virus-D, and mixed infections demonstrated that the observed differences are a result of cis-acting functions.

摘要

通过结合变性凝胶和核酸杂交探针使用各种化学和酶处理方法，对未整合的脾坏死病毒DNA的结构进行了表征。在病毒感染过程中，病毒DNA的主要种类是一种线性双链分子，其中核糖核苷酸与DNA共价连接。负链和正链中的大多数都是连续的。核糖核苷酸连接似乎相对较短，并且核糖核苷酸连接的碱基组成和分布是异质的。平均而言，负链中的核糖核苷酸连接比正链中的少。含有核糖核苷酸连接的病毒DNA在DNA转染试验中具有感染性。脾坏死病毒DNA的结构与施密特-鲁平劳氏肉瘤病毒-D不同，混合感染表明观察到的差异是顺式作用功能的结果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/ce7f6c2e997e/jvirol00171-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/38018ecfe22d/jvirol00171-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/2f9ad9678cb1/jvirol00171-0143-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/b29c3b0b3592/jvirol00171-0145-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/d0801ea005d8/jvirol00171-0147-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/c8d3b59c088f/jvirol00171-0148-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/68ef104b272f/jvirol00171-0148-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/c3f1fee5410d/jvirol00171-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/0333e106da7d/jvirol00171-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/ce7f6c2e997e/jvirol00171-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/38018ecfe22d/jvirol00171-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/2f9ad9678cb1/jvirol00171-0143-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/b29c3b0b3592/jvirol00171-0145-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/d0801ea005d8/jvirol00171-0147-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/c8d3b59c088f/jvirol00171-0148-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/68ef104b272f/jvirol00171-0148-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/c3f1fee5410d/jvirol00171-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/0333e106da7d/jvirol00171-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae29/288639/ce7f6c2e997e/jvirol00171-0152-a.jpg

相似文献

1
Ribonucleotides in unintegrated linear spleen necrosis virus DNA.未整合的线性脾坏死病毒DNA中的核糖核苷酸。
J Virol. 1980 Mar;33(3):1058-73. doi: 10.1128/JVI.33.3.1058-1073.1980.
2
Quantitative determination and location of newly synthesized virus-specific ribonucleic acid in chicken cells infected with Rous sarcoma virus.罗氏肉瘤病毒感染的鸡细胞中新合成的病毒特异性核糖核酸的定量测定与定位
J Virol. 1973 May;11(5):761-74. doi: 10.1128/JVI.11.5.761-774.1973.
3
Cell killing by spleen necrosis virus is correlated with a transient accumulation of spleen necrosis virus DNA.脾脏坏死病毒导致的细胞杀伤与脾脏坏死病毒DNA的短暂积累相关。
J Virol. 1979 Aug;31(2):376-88. doi: 10.1128/JVI.31.2.376-388.1979.
4
Localization of the ribonucleotide sites in rat liver mitochondrial deoxyribonucleic acid.大鼠肝脏线粒体脱氧核糖核酸中核糖核苷酸位点的定位
Biochem J. 1978 Jan 1;169(1):79-85. doi: 10.1042/bj1690079.
5
Ribonucleotides covalently linked to deoxyribonucleic acid in T4 bacteriophage.核糖核苷酸与T4噬菌体中的脱氧核糖核酸共价连接。
J Virol. 1972 Nov;10(5):902-8. doi: 10.1128/JVI.10.5.902-908.1972.
6
Single-stranded regions on unintegrated avian retrovirus DNA.未整合的禽逆转录病毒DNA上的单链区域
J Virol. 1982 Oct;44(1):47-53. doi: 10.1128/JVI.44.1.47-53.1982.
7
[Fragments of linear unintegrated Rous sarcoma virus DNA, resulting from digestion with SmaI restriction endonuclease].[线性未整合劳斯肉瘤病毒DNA片段，由SmaI限制性内切核酸酶消化产生]
C R Seances Acad Sci D. 1979 Nov 26;289(14):1021-4.
8
Ribonucleotides in newly synthesized DNA of herpes simplex virus.单纯疱疹病毒新合成DNA中的核糖核苷酸。
Virology. 1974 Sep;61(1):87-99. doi: 10.1016/0042-6822(74)90244-x.
9
Formation and structure of infectious DNA of spleen necrosis virus.脾坏死病毒感染性DNA的形成与结构
J Virol. 1977 Jan;21(1):119-30. doi: 10.1128/JVI.21.1.119-130.1977.
10
High resolution autoradiographic study of the kinetics of development of Rous sarcoma virus (Schmidt-Ruppin strain). V. Estimation of the time of synthesis of virus-associated DNA.劳氏肉瘤病毒（施密特-鲁平株）发育动力学的高分辨率放射自显影研究。V. 病毒相关DNA合成时间的估计
J Natl Cancer Inst. 1973 Dec;51(6):1927-33. doi: 10.1093/jnci/51.6.1927.

引用本文的文献

1
Partial characterisation of different classes of viral DNA, and kinetics of DNA synthesis in turnip protoplasts infected with cauliflower mosaic virus.不同类型病毒 DNA 的部分特征，以及芜菁原生质体感染花椰菜花叶病毒后 DNA 合成的动力学。
Plant Mol Biol. 1985 Jan;5(1):25-34. doi: 10.1007/BF00017870.
2
Spontaneous changes in nucleotide sequence in proviruses of spleen necrosis virus, an avian retrovirus.脾脏坏死病毒（一种禽逆转录病毒）原病毒核苷酸序列的自发变化。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1230-4. doi: 10.1073/pnas.79.4.1230.
3
Structure and dimorphism of c-rel (turkey), the cellular homolog to the oncogene of reticuloendotheliosis virus strain T.

本文引用的文献

1
Separation of native and denatured DNA, RNA and hybrid on sodium iodide gradients.在碘化钠梯度上分离天然和变性的DNA、RNA及杂交体。
FEBS Lett. 1972 Oct 15;27(1):19-22. doi: 10.1016/0014-5793(72)80399-5.
2
Correlation between cell killing and massive second-round superinfection by members of some subgroups of avian leukosis virus.禽白血病病毒某些亚群成员的细胞杀伤与大规模第二轮超级感染之间的相关性。
J Virol. 1980 Jan;33(1):494-506. doi: 10.1128/JVI.33.1.494-506.1980.
3
Replication of reticuloendotheliosis viruses in cell culture: acute infection.
c-rel（火鸡）的结构与二态性，它是网状内皮组织增生症病毒T株致癌基因的细胞同源物。
J Virol. 1984 Feb;49(2):521-9. doi: 10.1128/JVI.49.2.521-529.1984.
4
Establishment of infection by spleen necrosis virus: inhibition in stationary cells and the role of secondary infection.脾脏坏死病毒感染的建立：静止细胞中的抑制作用及二次感染的作用
J Virol. 1982 Jan;41(1):183-91. doi: 10.1128/JVI.41.1.183-191.1982.
5
Characterization of reticuloendotheliosis virus strain T DNA and isolation of a novel variant of reticuloendotheliosis virus strain T by molecular cloning.禽网状内皮组织增殖病病毒T株DNA的特性分析及通过分子克隆分离出禽网状内皮组织增殖病病毒T株的一种新型变体
J Virol. 1981 Dec;40(3):800-11. doi: 10.1128/JVI.40.3.800-811.1981.
6
Synthesis of plus strands of retroviral DNA in cells infected with avian sarcoma virus and mouse mammary tumor virus.在感染禽肉瘤病毒和小鼠乳腺肿瘤病毒的细胞中逆转录病毒DNA正链的合成。
J Virol. 1981 Jan;37(1):127-38. doi: 10.1128/JVI.37.1.127-138.1981.
7
Viral DNA synthesized in vitro by avian retrovirus particles permeabilized with melittin. II. Evidence for a strand displacement mechanism in plus-strand synthesis.用蜂毒肽通透化的禽逆转录病毒颗粒在体外合成的病毒DNA。II. 正链合成中链置换机制的证据。
J Virol. 1981 Jan;37(1):117-26. doi: 10.1128/JVI.37.1.117-126.1981.
8
On the early emergence of reverse transcription: theoretical basis and experimental evidence.关于逆转录的早期出现：理论基础与实验证据。
J Mol Evol. 1992 Dec;35(6):524-36. doi: 10.1007/BF00160213.
网状内皮组织增殖症病毒在细胞培养中的复制：急性感染
J Virol. 1974 Feb;13(2):291-7. doi: 10.1128/JVI.13.2.291-297.1974.
4
A new technique for the assay of infectivity of human adenovirus 5 DNA.一种检测人腺病毒5型DNA感染性的新技术。
Virology. 1973 Apr;52(2):456-67. doi: 10.1016/0042-6822(73)90341-3.
5
RNA in coliphage T5.大肠杆菌噬菌体T5中的核糖核酸
Nature. 1973 Mar 30;242(5396):327-9. doi: 10.1038/242327a0.
6
Ribonucleotides covalently linked to deoxyribonucleic acid in T4 bacteriophage.核糖核苷酸与T4噬菌体中的脱氧核糖核酸共价连接。
J Virol. 1972 Nov;10(5):902-8. doi: 10.1128/JVI.10.5.902-908.1972.
7
Reticuloendotheliosis virus nucleic acid sequences in cellular DNA.细胞DNA中的网状内皮组织增殖症病毒核酸序列。
J Virol. 1974 Nov;14(5):1179-88. doi: 10.1128/JVI.14.5.1179-1188.1974.
8
Infectious rous sarcoma virus and reticuloendotheliosis virus DNAs.传染性劳氏肉瘤病毒和网状内皮组织增殖症病毒DNA
J Virol. 1974 Nov;14(5):1132-41. doi: 10.1128/JVI.14.5.1132-1141.1974.
9
Ribonucleotides linked to DNA of herpes simplex virus type 1.与1型单纯疱疹病毒DNA相连的核糖核苷酸。
J Virol. 1974 Jun;13(6):1162-8. doi: 10.1128/JVI.13.6.1162-1168.1974.
10
Base-sequence relationships between avian ribonucleic acid endogenous and sarcoma viruses assayed by competitive ribonucleic acid-deoxyribonucleic acid hybridization.通过竞争性核糖核酸-脱氧核糖核酸杂交分析禽核糖核酸内源病毒和肉瘤病毒之间的碱基序列关系。
Biochemistry. 1974 Mar 26;13(7):1549-54. doi: 10.1021/bi00704a035.