Sangwan Parveen, Wu Dong Yang
CSIRO Materials Science and Engineering, Victoria 3168, Australia.
Macromol Biosci. 2008 Apr 9;8(4):304-15. doi: 10.1002/mabi.200700317.
Molecular ecological techniques for direct identification of microbes involved in PLA degradation under aerobic composting conditions are described. Gene sequences from genera Paecilomyces, Thermomonospora, and Thermopolyspora were most abundant in the compost samples. Members of these phylogenetic lineages are therefore likely to play an important role in PLA degradation. The use of molecular ecological techniques to design cultivation strategies may also provide a new tool for identification and investigation of biodegradation mechanisms and for future development of efficient biological treatment or recycling processes for PLA and other biodegradable polymers.
本文描述了在好氧堆肥条件下直接鉴定参与聚乳酸(PLA)降解的微生物的分子生态学技术。在堆肥样品中,拟青霉属、嗜热单孢菌属和嗜热多孢菌属的基因序列最为丰富。因此,这些系统发育谱系的成员可能在PLA降解中发挥重要作用。利用分子生态学技术设计培养策略,也可能为鉴定和研究生物降解机制,以及未来开发PLA和其他可生物降解聚合物的高效生物处理或回收工艺提供一种新工具。