Mitsui Chemicals, Inc., 1-5-2 Higashi-Shimbashi, Minato-Ku, Tokyo, 105-7122, Japan.
Mitsui Chemicals, Inc., 1144 Togo, Mobara, Chiba, 297-0017, Japan.
Arch Microbiol. 2022 Sep 3;204(10):599. doi: 10.1007/s00203-022-03144-w.
A new Nocardiopsis species that degrades polylactic acid (PLA) was isolated from pig dung-based compost from a municipal composting facility in Japan. To obtain strains capable of efficient PLA degradation, the effect of non-enzymatic degradation of PLA was minimized by maintaining the temperature at or below 37 °C. Screening 15 animal waste-based compost samples, consisting of pig dung, cow dung, horse dung, or chicken droppings, revealed that compost derived from pig dung was most efficient for degradation of PLA films. Hence, pig waste-based compost was used to isolate PLA-degrading microorganisms by screening for PLA-degrading microorganisms in compost using an agar plate-based method in which an emulsifier was omitted to avoid selecting strains that assimilated the emulsifier instead of PLA in the medium. Repeated enrichment obtained six strains. The one that exhibited stable PLA degradation on agar plates was subjected to genomic analysis and identified as Nocardiopsis chromatogenes, an actinomycete.
从日本一个城市堆肥设施的猪粪堆肥中分离到一种能够降解聚乳酸（PLA）的新诺卡氏菌属物种。为了获得能够有效降解 PLA 的菌株，通过将温度保持在 37°C 或以下，将 PLA 的非酶降解的影响降到最低。筛选了 15 种动物粪便堆肥样本，包括猪粪、牛粪、马粪或鸡粪，结果表明，猪粪堆肥最有利于 PLA 薄膜的降解。因此，使用猪粪堆肥通过在堆肥中筛选 PLA 降解微生物，来分离 PLA 降解微生物，采用的琼脂平板法中省略了乳化剂，以避免选择那些在培养基中同化乳化剂而不是 PLA 的菌株。重复富集得到了 6 株菌。在琼脂平板上表现出稳定 PLA 降解的一株菌被进行基因组分析，并鉴定为放线菌色诺卡氏菌。
