西尼罗河病毒在加勒比地区的首次分离。

First isolation of West Nile virus in the Caribbean.

作者信息

Barrera Roberto, Hunsperger Elizabeth, Muñoz-Jordán Jorge L, Amador Manuel, Diaz Annette, Smith Joshua, Bessoff Kovi, Beltran Manuela, Vergne Edgardo, Verduin Mark, Lambert Amy, Sun Wellington

机构信息

Dengue Branch, DVBID, Centers for Disease Control and Prevention, 1324 Calle Cañada, San Juan, Puerto Rico.

出版信息

Am J Trop Med Hyg. 2008 Apr;78(4):666-8.

PMID:18385366

Abstract

A sentinel chicken program for West Nile virus (WNV) surveillance was initiated in July 2006 in eastern Puerto Rico, yielding the first seroconversions on June 4, 2007. WNV was isolated from sentinel chicken serum and mosquito pools (Culex nigripalpus, Culex bahamensis) for the first time in Tropical America. Preliminary sequence analysis of the prM and E genes revealed a 1-amino acid difference (V159A) between the Puerto Rican 2007 and the NY99. This mutation has been observed in the current dominant clade circulating in the United States. Sentinel chicken surveillance was a useful tool for the detection of West Nile virus in the tropics.

摘要

2006年7月在波多黎各东部启动了一项用于西尼罗河病毒（WNV）监测的哨兵鸡计划，于2007年6月4日首次出现血清转化。西尼罗河病毒首次在热带美洲从哨兵鸡血清和蚊虫样本（黑须库蚊、巴哈马库蚊）中分离出来。对prM和E基因的初步序列分析显示，2007年波多黎各毒株与NY99毒株之间存在1个氨基酸差异（V159A）。在美国当前流行的主要进化枝中已观察到这种突变。哨兵鸡监测是在热带地区检测西尼罗河病毒的一种有用工具。