Chang Hui-Hsiang, Chen Chin-Shun, Lin Jin-Yuarn
Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung , 40227, Taiwan, ROC.
Lipids. 2008 Jun;43(6):499-506. doi: 10.1007/s11745-008-3171-8. Epub 2008 Apr 2.
To evaluate the anti-inflammatory effects of different dietary oils on ovalbumin-sensitized and -challenged mice. Experimental BALB/c mice were fed with different diets containing 5% corn oil [rich in linoleic acid, 18:2n-6 polyunsaturated fatty acids (PUFA), as a control diet], 5% perilla oil (rich in alpha-linolenic acid, 18:3n-3 PUFA) or 5% compound oil containing 50% corn oil and 50% perilla oil, for 5 consecutive weeks. The leukocyte count, inflammatory mediators, and cytokine levels, including proinflammatory and Th1/Th2 cytokines in the bronchoalveolar lavage fluid (BALF) from the mice were determined. The results showed that 5% compound oil administration significantly (P < 0.05) decreased eosinophilic infiltration. Dietary perilla oil could not significantly (P > 0.05) decrease the eosinophil accumulation or the secretions of inflammatory mediators such as prostaglandin E2 (PGE2), histamine, nitric oxide and eotaxin. However, dietary perilla oil significantly (P < 0.05) reduced proinflammatory cytokine (TNF-alpha, IL-1beta and IL-6) and Th1 cytokine (IFN-gamma and IL-2) production. The production of Th2 cytokine IL-10, but not IL-4 and IL-5, was also significantly inhibited by perilla oil administration. The results suggest that dietary perilla oil might alleviate inflammation via decreasing the secretion of pro-inflammatory cytokines in BALF, but failed to regulate the Th1/Th2 balance toward Th1 pole during the Th2-skewed allergic airway inflammation.
为评估不同食用油对卵清蛋白致敏和激发小鼠的抗炎作用。将实验性BALB/c小鼠连续5周喂食含有5%玉米油(富含亚油酸,18:2n-6多不饱和脂肪酸(PUFA),作为对照饮食)、5%紫苏油(富含α-亚麻酸,18:3n-3 PUFA)或5%由50%玉米油和50%紫苏油组成的复合油的不同饮食。测定小鼠支气管肺泡灌洗液(BALF)中的白细胞计数、炎症介质和细胞因子水平,包括促炎细胞因子和Th1/Th2细胞因子。结果显示,给予5%复合油可显著(P<0.05)减少嗜酸性粒细胞浸润。食用紫苏油不能显著(P>0.05)减少嗜酸性粒细胞聚集或炎症介质如前列腺素E2(PGE2)、组胺、一氧化氮和嗜酸性粒细胞趋化因子的分泌。然而,食用紫苏油可显著(P<0.05)降低促炎细胞因子(TNF-α、IL-1β和IL-6)和Th1细胞因子(IFN-γ和IL-2)的产生。给予紫苏油也显著抑制了Th2细胞因子IL-10的产生,但未抑制IL-4和IL-5的产生。结果表明,食用紫苏油可能通过减少BALF中促炎细胞因子的分泌来减轻炎症,但在Th2型过敏性气道炎症期间未能将Th1/Th2平衡调节至Th1极。