CBQF Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.
Molecules. 2022 Aug 4;27(15):4958. doi: 10.3390/molecules27154958.
In recent years, pomegranate oil has obtained more attention due to its content of conjugated linolenic acids and possible application in the prevention of many diseases. The purpose of this work was to evaluate the potential ability of pomegranate oil to modulate obesity-related metabolism and immune response using in vitro models. In this regard, pomegranate oil was characterized in terms of fatty acids profile, tocopherols and phytosterols, and antioxidant capacity. After evaluation of the safety profile, pomegranate oil's capacity to modulate obesity-related metabolism was evaluated through adipolysis and adipokines secretion quantification in 3T3-L1 differentiated adipocytes and hepatic lipid accumulation assay in Hep G2 hepatocytes. The immunomodulatory activity was evaluated in Caco-2 cells by quantification of pro-inflammatory cytokines IL-6, IL-8, and TNF-α. This oil showed high antioxidant capacity and was mainly composed of conjugated fatty acid, namely punicic acid. Its chemical composition was responsible for its capacity to reduce the lipid accumulation in Hep G2 cells and 3T3-L1 differentiated adipocytes. In short, pomegranate oil shows great potential for the development of functional foods and nutraceuticals targeting obesity.
近年来，由于其共轭亚油酸的含量以及在预防许多疾病方面的潜在应用，石榴籽油受到了更多的关注。本工作的目的是使用体外模型评估石榴籽油调节肥胖相关代谢和免疫反应的潜在能力。为此，对石榴籽油的脂肪酸组成、生育酚和植物甾醇以及抗氧化能力进行了表征。在评估安全性概况后，通过定量测定 3T3-L1 分化脂肪细胞中的脂肪分解和 adipokines 分泌以及 Hep G2 肝细胞中的肝脂质积累来评估石榴籽油调节肥胖相关代谢的能力。通过定量测定促炎细胞因子 IL-6、IL-8 和 TNF-α，在 Caco-2 细胞中评估其免疫调节活性。这种油具有很高的抗氧化能力，主要由共轭脂肪酸，即 punicic 酸组成。其化学成分是其减少 Hep G2 细胞和 3T3-L1 分化脂肪细胞中脂质积累的能力的原因。总之，石榴籽油在开发针对肥胖的功能性食品和营养保健品方面具有很大的潜力。
