Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand.
Division of Physiology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand.
Molecules. 2021 Nov 9;26(22):6757. doi: 10.3390/molecules26226757.
Industrially, after the removal of oil from perilla seeds (PS) by screw-type compression, the large quantities of residual perilla seed meal (PSM) becomes non-valuable waste. Therefore, to increase the health value and price of PS and PSM, we focused on the biological effects of perilla seed oil (PSO) and rosmarinic acid-rich fraction (RA-RF) extracted from PSM for their role in preventing oxidative stress and inflammation caused by TNF-α exposure in an A549 lung adenocarcinoma culture model. The A549 cells were pretreated with PSO or RA-RF and followed by TNF-α treatment. We found that PSO and RA-RF were not toxic to TNF-α-induced A549 cells. Both extracts significantly decreased the generation of reactive oxygen species (ROS) in this cell line. The mRNA expression levels of IL-1β, IL-6, IL-8, TNF-α, and COX-2 were significantly decreased by the treatment of PSO and RA-RF. The Western blot indicated that the expression of MnSOD, FOXO1, and NF-κB and phosphorylation of JNK were also significantly diminished by PSO and RA-RF treatment. The results demonstrated that PSO and RA-RF act as antioxidants to scavenge TNF-α induced ROS levels, resulting in decreased the expression of MnSOD, FOXO1, NF-κB and JNK signaling pathway in a human lung cell culture exposed to TNF-α.
工业上，从紫苏种子（PS）中通过螺旋式压缩去除油后，大量的剩余紫苏籽粉（PSM）成为无价值的废物。因此，为了提高 PS 和 PSM 的健康价值和价格，我们专注于从 PSM 中提取的紫苏籽油（PSO）和迷迭香酸丰富馏分（RA-RF）的生物学效应，研究它们在预防 TNF-α 暴露引起的氧化应激和炎症中的作用在 A549 肺腺癌细胞培养模型中。用 PSO 或 RA-RF 预处理 A549 细胞，然后用 TNF-α 处理。我们发现 PSO 和 RA-RF 对 TNF-α 诱导的 A549 细胞没有毒性。这两种提取物都能显著降低该细胞系中活性氧（ROS）的产生。PSO 和 RA-RF 处理还显著降低了 IL-1β、IL-6、IL-8、TNF-α 和 COX-2 的 mRNA 表达水平。Western blot 表明，PSO 和 RA-RF 处理还显著降低了 MnSOD、FOXO1、NF-κB 和 JNK 的表达以及 JNK 的磷酸化。结果表明，PSO 和 RA-RF 作为抗氧化剂清除 TNF-α 诱导的 ROS 水平，从而降低 TNF-α 暴露的人肺细胞培养物中 MnSOD、FOXO1、NF-κB 和 JNK 信号通路的表达。
